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Digestion and metabolism of carbohydrates in fish

Abro, Rani (2014). Digestion and metabolism of carbohydrates in fish. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2014:1
ISBN 978-91-576-7950-5
eISBN 978-91-576-7951-2
[Doctoral thesis]

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Abstract

This thesis deals with the digestion and metabolism of carbohydrates in Arctic charr, Eurasian perch and tilapia. Two sources of carbohydrates, native starch (wheat) and chitin (zygomycete biomass), were evaluated.

Gut tissue of Arctic charr displayed significant chitinase activity, of both endo- and exo-chitinase forms. Moreover, the distribution pattern along the gastrointestinal tract of Arctic charr differed between endo-chitinase and exo-chitinase. The endo-chitinase activity in stomach tissue and in the distal intestine was several hundred-fold higher than the exo-chitinase activity in stomach tissue. The greatest exo-chitinase activity was found in the distal intestine fed a zygomycete-based diet. Disturbed intestinal integrity and increased uptake rate of the amino acid lysine were observed in the distal, but not proximal, intestine of fish fed the zygomycete-based feed.

A ¹HNMR metabolomics approach revealed no differences in metabolic profile in liver tissues of Arctic charr fingerlings fed a zygomycete-based diet and a fish-meal based diet.

The inclusion of wheat starch did not affect α-amylase activity in gut tissue of Arctic charr and Eurasian perch. Overall, α-amylase activity was correlated with the trends obtained for starch digestibility. The apparent digestibility (AD) of crude protein, starch, crude fat and energy differed between the fish species, with on average higher values for all parameters in Eurasian perch than in Arctic charr. Within fish species, dietary starch level had no effect on AD of dry matter, crude protein, crude fat and energy.

Studies of the metabolic response to wheat starch inclusion in Arctic charr and tilapia using ¹HNMR base metabolomics indicated metabolic effects in tilapia, while inclusion of starch in the diet of Arctic charr resulted in partial or negligible metabolism effects. Thus there are species-related differences in the metabolic response to dietary starch inclusion.

Authors/Creators:Abro, Rani
Title:Digestion and metabolism of carbohydrates in fish
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :10 January 2014
Volume:2014:1
Number of Pages:58
Papers/manuscripts:
NumberReferences
I.Abro, R., Sundell, K., Sandblom, E., Sundh, H., Brännäs, E., Kiessling, A., Lindberg, J. E., Lundh, T. (2013). Zygomycete biomass in the diet enhances intestinal chitinolytic activities and increases leakiness in the intestinal epithelia of Arctic charr (Salvelinus alpinus). Comparative Biochemistry and Physiology B (Submitted).
II.Abro, R., Moazzami, A. A., Lindberg, J. E., Lundh, T. (2013). Metabolic insights in Arctic charr (Salvelinus alpinus) fed zygomycetes and fish meal diets as assessed in liver using nuclear magnetic resonance (NMR) technology. International Aquatic Research (Submitted).
III.Abro, R., Moazzami, A. A., Lindberg, J. E., Lundh, T. (2013). NMR-based metabolomics reveals dietary effects in liver extracts of Arctic charr (Salvelinus alpinus) and tilapia (Oreochromis mossambicus) fed different levels of starch (Manuscript).
IV.Abro, R., Lundh, T., Lindberg, J. E. (2013). Effect of dietary starch inclusion rate on digestibility and amylase activity of Arctic charr (Salvelinus alpinus) and Eurasian perch (Perca fluviatilis). Journal of Aquaculture Research and Development [5: 209 doi:10.4172/2155-9546.1000209].
Place of Publication:Uppsala
Publisher:Dept. of Animal Nutrition and Management, Swedish University of Agricltural Sciences
ISBN for printed version:978-91-576-7950-5
ISBN for electronic version:978-91-576-7951-2
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L51 Animal physiology - Nutrition
M Aquatic sciences and fisheries > M12 Aquaculture production
Q Food science > Q54 Feed composition
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Medical Bioscience
Agrovoc terms:chars, salvelinus, perch, perca fluviatilis, tilapia, diet, fish feeding, carbohydrates, wheats, starch, chitin, zygomycetes, carbohydrate metabolism, enzymes, amylases, animal physiology
Keywords:carbohydrates, chitin, metabolism, fish, diet, metabolites, wheat starch, enzyme, zygomycete
URN:NBN:urn:nbn:se:slu:epsilon-e-1749
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1749
ID Code:10974
Department:(VH) > Dept. of Animal Nutrition and Management
Deposited By: Mrs Rani Abro
Deposited On:13 Jan 2014 08:05
Metadata Last Modified:02 Dec 2014 11:04

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