Abstract

To date, there are no validated internal reference genes for the normalization of RT-qPCR data from virus infection experiments with pollinating insects. In this study we evaluated the stability of five candidate internal reference genes: elongation factor-1 -alpha (ELF1 alpha), peptidylprolyl isomerase A (PPIA), 60S ribosomal protein L23 (RPL23), TATA-binding protein (TBP) and polyubiquitin (UBI), in relation to Israeli acute paralysis virus (IAPV) infection of Bombus terrestris. We investigated the stability of these genes: in whole bodies and individual body parts, as well as in whole bodies collected at different time intervals after infection with IAPV. Our data identified PPIA as the single, most-optimal internal reference gene and the combination of PPAI-RPL23-UBI as a fully-sufficient multiple internal reference genes set for IAPV infection experiments in B. terrestris. (C) 2013 Elsevier Inc. All rights reserved.

Keywords

Gene expression; Bee virus; RT-qPCR; Dicistroviruses; qBase(PLUS)

Published in

Journal of Invertebrate Pathology
2014, Volume: 115, pages: 76-79
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE

SLU Authors

• Rodrigues de Miranda, Joachim

  • Department of Ecology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Biochemistry and Molecular Biology


Publication identifier

DOI: https://doi.org/10.1016/j.jip.2013.10.011

Permanent link to this page (URI)

https://res.slu.se/id/publ/63764