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Molecular characterization and prevalence of hepatitis E virus in Swedish wild animals

a zoonotic perspective

Lin, Jay (2015). Molecular characterization and prevalence of hepatitis E virus in Swedish wild animals. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2015:86
ISBN 978-91-576-8370-0
eISBN 978-91-576-8371-7
[Doctoral thesis]

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Abstract

Observation of chronic hepatitis E virus (HEV) in immunosuppressed patients, and unexplained high hepatitis E virus (HEV) prevalence in the human population raises public health concern. The aim of this thesis is to molecular characterize and investigate the prevalence of HEV in Swedish wild life and their association with HEV transmission to humans. A novel virus was detected in a sample from a Swedish moose (Alces alces). The genome was highly divergent with sequence identity of 30-60% to other HEVs. Genome sequence and phylogenetic analysis showed closest relationship with HEV genotypes1-7 (gt1-7). In addition, three open reading frames (ORFs) was also detected, and all these observed properties suggested the virus as a member of the Hepeviridae family. Markers for ongoing (HEV RNA) and/or past HEV infection (anti-HEV) was demonstrated in 67 (29%) of 231 Swedish moose samples collected from various Swedish provinces. Thus, moose are frequently infected with HEV. Its closest similarity with the HEV gt1-7 group, which includes strains that also infects humans, may indicate a potential for zoonotic transmission of this HEV. A survey detected HEV markers in the wild life, which included samples from wild boars (Sus scrofa) and different deer species, fallow deer (Darna darna), red deer (Cervus elaphus), roe deer (Capreolus capreolus) and moose (Alces alces). These markers were ongoing and/or past infections, and were found in 53 (22%) out of 245 animal samples. The viral nucleic acid sequences strains were sequenced and compared with autochthonous Swedish human HEV cases, of whom three were found infected with strains similar to wild boar strains. These results indicate that Swedish wild animals are often infected with HEV and may be an important source of HEV transmission to humans who come into contact with wild animals or eat game meat. The introduction of a single amplicon PCR of near complete HEV genomes enabled the identification of possible virulence marker, and the detection of possible recombination events between Swedish swine and wild boar, and that there may have been zoonotic transmission of HEV strains between Spain and France.

Authors/Creators:Lin, Jay
Title:Molecular characterization and prevalence of hepatitis E virus in Swedish wild animals
Subtitle:a zoonotic perspective
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :11 September 2015
Depositing date:11 September 2015
Volume:2015:86
Number of Pages:104
Papers/manuscripts:
NumberReferences
ILin J., Norder H., Uhlhorn H., Belák, S. and Widén, F. (2013). Novel Hepatitis E virus found in Swedish moose. The Journal of General Virology 95(3), 557-570.
IILin J., Karlsson, M., Olofson A. S., Belák S., Malmsten J., Dalin A. M., Widén F and Norder H. (2015). High prevalence of Hepatitis E Virus in Swedish moose- a phylogenetic characterization and comparison of the virus from different regions. PLoS One 10(4), e0122102.
IIILin J., Norder H., Belák S., and Widén F. (2015). Near complete single PCR amplification of porcine Hepatitis E virus genomes; characterization of genomic regions of potential recombination and markers of zoonosis and virulence elements (manuscript).
IVRoth A., Lin J., Magnius L., Karlsson M., Bélak S., Widén F. and Norder H. (2015) Swedish wild ungulates are commonly infected with hepatitis E virus (manuscript).
Place of Publication:Uppsala
Publisher:Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8370-0
ISBN for electronic version:978-91-576-8371-7
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L50 Animal physiology and biochemistry
L Animal production > L73 Animal diseases
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Evolutionary Biology
Agrovoc terms:wild animals, elks, deer, wild boar, swine, hepatitis, zoonoses, pathogenesis, virulence, recombination, pcr, elisa, molecular biology
Keywords:Hepatitis E virus, wild life, deer, wild boar, moose, swine, zoonosis, recombination, virulence, classification, molecular tracing, epidemiology
URN:NBN:urn:nbn:se:slu:epsilon-e-2927
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2927
ID Code:12604
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: Mr Jay Lin
Deposited On:14 Sep 2015 11:08
Metadata Last Modified:29 Jan 2016 09:52

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