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Root rot of pea caused by Aphanomyces euteiches

calcium-dependent soil suppressiveness, molecular detection and population structure

Heyman, Fredrik (2008). Root rot of pea caused by Aphanomyces euteiches. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2008:24
ISBN 978-91-85913-57-2
[Doctoral thesis]

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Abstract

Aphanomyces root rot, caused by the oomycete Aphanomyces euteiches, is the most important and destructive disease of pea. Since the inoculum is persistent in the soil, and no resistant cultivars or means of chemical control are available, the disease is difficult to manage. The occurrence of soil suppressiveness against the disease was previously reported, and the main aim of this study was to understand the mechanism. By analysing chemical data for naturally infested soil samples from a large number of fields, we found that disease suppression was correlated to the Ca content of the soil. This was also true for artificially inoculated soil samples. Suppressiveness was only inconsistently affected by soil sterilisation, and was concluded to be abiotic in nature. Amendments of non-suppressive soils with both neutral and alkaline Ca salts strongly reduced disease severity. In contrast, amendment of suppressive soils with NaHCO3 solutions lowered the content of soluble Ca, increased pH and made the soil less suppressive. It was concluded that high concentrations of Ca in the soil solution was a major factor causing suppressiveness. The effect of Ca in the field was evaluated by adding a neutral Ca salt, CaSO4 in a naturally infested field trial. A clear delay in the onset of disease symptoms was seen at all doses evaluated. A quantitative molecular detection method based on quantitative PCR was developed and compared with the greenhouse biotest traditionally used to detect the pathogen in soil samples. Using the qPCR assay, it was possible to detect and quantify the pathogen at concentrations under 10 oospores g soil-1. The Swedish population of A. euteiches was analysed using co-dominant DNA sequence markers. It was found that the population was dominated by a globally distributed genotype, but that distinctly different genotypes also exist. These results were interpreted as evidence of a predominantly clonal mode of reproduction, but also of occasional sexual recombination.

Authors/Creators:Heyman, Fredrik
Title:Root rot of pea caused by Aphanomyces euteiches
Subtitle:calcium-dependent soil suppressiveness, molecular detection and population structure
Year of publishing :2008
Volume:2008:24
Number of Pages:38
Papers/manuscripts:
NumberReferences
ALLI Heyman F, Lindahl, B, Persson L, Wikström M, Stenlid J. (2007). Calcium concentrations of soil affect suppressiveness against Aphanomyces root rot of pea. Soil Biology and Biochemistry vol (39), 2222-2229. II Heyman F, Wikström M, Persson L, Lindahl B, Stenlid J. Effect of calcium sulphate on Aphanomyces root rot of pea in field experiments (manuscript). III Heyman F, Almquist C, Jonsson A, Lindahl B, Stenlid J. Evaluation of a quantitative PCR method for detection and quantification of Aphanomyces euteiches in soil samples (manuscript). IV Heyman F, Lindahl B, Wikström M, Stenlid J. Analysis of the Swedish population of Aphanomyces euteiches using co-dominant markers (manuscript).
Place of Publication:Uppsala
ISBN for printed version:978-91-85913-57-2
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:aphanomyces euteiches, mastigomycotina, genotypes, pisum sativum, root rots, soil chemicophysical properties, calcium, pcr
Keywords:Aphanomyces euteiches, oomycetes, pea, soil suppressiveness, calcium, zoospores, detection, population
URN:NBN:urn:nbn:se:slu:epsilon-2146
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-2146
ID Code:1652
Department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: Fredrik Heyman
Deposited On:28 Mar 2008 00:00
Metadata Last Modified:02 Dec 2014 10:13

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