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studies of Hymenoscyphus pseudoalbidus, Fraxinus excelsior and Pseudomonas brassicacearum MA250

Andersson, Pierre F. (2012). Secondary metabolites associated with plant disease, plant defense and biocontrol. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2012:52
ISBN 978-91-576-7699-3
[Doctoral thesis]

PDF (Doktorsavhandling)


Secondary metabolites of the fungal ash dieback pathogen Hymenoscyphus pseudoalbidus were investigated. Nine steroidal compounds were isolated from, or detected in, liquid cultures of the fungus. Three were the previously known compounds viridiol, viridin and demethoxyviridiol. Six were the previously unreported compounds 1-deoxyviridiol, 1-deoxy-2-demethylviridiol, 3-dihydrovirone, B-norviridin enol, Bnorviridiol lactone and 1β-hydroxy-2α-hydroasterogynin A. All of the compounds are structurally related to each other, although the three latter are B-norsteroids, which are rare in nature. Viridiol displayed phytotoxic activity to seedlings of the natural host Fraxinus excelsior. The amount of damage caused by viridiol was higher on ash seedlings of clones, classed as susceptible to H. pseudoalbidus. The metabolomes of resistant clones of F. excelsior were compared to those of susceptible clones. No differences were detected that were common to all clones within the groups. Metabolomes of ash seedlings treated with viridiol were compared to those of untreated ash seedlings. A number of significant changes in the concentrations of compounds were observed at the clone level. No change common to all resistant or susceptible clones was observed. Secondary metabolites from the bacterial biocontrol agent Pseudomonas brassicacearum MA250 were investigated. The known compounds piliferolide A and SB-253514, as well as, the previously unreported compound piliferoic acid A were isolated from the bacterium. The compounds displayed growth inhibition of the fungal pathogen Microdochium nivale at concentrations down to 180 μM, which indicates that they may contribute to the biocontrol effect of P. brassicacearum MA250 on M. nivale.

Authors/Creators:Andersson, Pierre F.
Title:Secondary metabolites associated with plant disease, plant defense and biocontrol
Subtitle:studies of Hymenoscyphus pseudoalbidus, Fraxinus excelsior and Pseudomonas brassicacearum MA250
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :22 August 2012
Number of Pages:58
I.Andersson, P.F., Johansson, S.B.K., Stenlid, J. & Broberg, A. (2010). Isolation, identification and necrotic activity of viridiol from Chalara fraxinea, the fungus responsible for dieback of ash. Forest pathology 40(1), 43-46.
II.Andersson, P.F., Bengtsson, S., Cleary, M.R., Stenlid, J. & Broberg, A. Viridin-like steroids from Hymenoscyphus pseudoalbidus (submitted to Phytochemistry, under revision).
III.Andersson, P.F., Bengtsson, S., Stenlid, J. & Broberg, A. (2012). Bnorsteroids from Hymenoscyphus pseudoalbidus. Molecules 17(7), 7769- 7781.
IV.Cleary, M.R., Andersson, P.F., Broberg, A., Daniel, G. & Stenlid, J. Clonal differences in sensitivity of Fraxinus excelsior to the phytotoxin viridiol associated with Hymenoscyphus pseudoalbidus, the causal agent of ash dieback (manuscript).
V.Andersson, P.F., Levenfors, J. & Broberg, A. (2012). Metabolites from Pseudomonas brassicacearum with activity against the pink snow mould causing pathogen Microdochium nivale. Biocontrol 57(3), 463-469.
Place of Publication:Uppsala
Publisher:Institutionen för kemi, Sveriges lantbruksuniversitet
ISBN for printed version:978-91-576-7699-3
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F60 Plant physiology and biochemistry
H Protection of plants and stored products > H20 Plant diseases
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 104 Chemical Sciences > Analytical Chemistry
(A) Swedish standard research categories 2011 > 1 Natural sciences > 104 Chemical Sciences > Organic Chemistry
Agrovoc terms:secondary metabolites, fraxinus excelsior, fungal diseases, chalara, pseudomonas, monographella nivalis, plant defense reactions, biological control, biological control agents, phytotoxins, steroids
Keywords:Chalara fraxinea, Hymenoscyphus pseudoalbidus, secondary metabolites, steroids, biocontrol, Microdochium nivale, Pseudomonas
Permanent URL:
ID Code:8996
Department:(NL, NJ) > Dept. of Chemistry (until 131231)
Deposited By: Mr Pierre F. Andersson
Deposited On:24 Aug 2012 08:57
Metadata Last Modified:02 Dec 2014 10:51

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