studies of Hymenoscyphus pseudoalbidus, Fraxinus excelsior and Pseudomonas brassicacearum MA250
Andersson, Pierre F.
Secondary metabolites associated with plant disease, plant defense and biocontrol.
Acta Universitatis agriculturae Sueciae, 1652-6880
Secondary metabolites of the fungal ash dieback pathogen Hymenoscyphus pseudoalbidus were investigated. Nine steroidal compounds were isolated from, or detected in, liquid cultures of the fungus. Three were the previously known compounds viridiol, viridin and demethoxyviridiol. Six were the previously unreported compounds 1-deoxyviridiol, 1-deoxy-2-demethylviridiol, 3-dihydrovirone, B-norviridin enol, Bnorviridiol lactone and 1β-hydroxy-2α-hydroasterogynin A. All of the compounds are
structurally related to each other, although the three latter are B-norsteroids, which are rare in nature. Viridiol displayed phytotoxic activity to seedlings of the natural host Fraxinus excelsior. The amount of damage caused by viridiol was higher on ash seedlings of clones, classed as susceptible to H. pseudoalbidus.
The metabolomes of resistant clones of F. excelsior were compared to those of susceptible clones. No differences were detected that were common to all clones within
the groups. Metabolomes of ash seedlings treated with viridiol were compared to those of untreated ash seedlings. A number of significant changes in the concentrations of
compounds were observed at the clone level. No change common to all resistant or susceptible clones was observed.
Secondary metabolites from the bacterial biocontrol agent Pseudomonas brassicacearum MA250 were investigated. The known compounds piliferolide A and SB-253514, as well as, the previously unreported compound piliferoic acid A were
isolated from the bacterium. The compounds displayed growth inhibition of the fungal pathogen Microdochium nivale at concentrations down to 180 μM, which indicates that
they may contribute to the biocontrol effect of P. brassicacearum MA250 on M. nivale.
|Authors/Creators:||Andersson, Pierre F.|
|Title:||Secondary metabolites associated with plant disease, plant defense and biocontrol|
|Subtitle:||studies of Hymenoscyphus pseudoalbidus, Fraxinus excelsior and Pseudomonas brassicacearum MA250|
|Series/Journal:||Acta Universitatis agriculturae Sueciae (1652-6880)|
|Year of publishing :||22 August 2012|
|Number of Pages:||58|
|Place of Publication:||Uppsala|
|Publisher:||Institutionen för kemi, Sveriges lantbruksuniversitet|
|ISBN for printed version:||978-91-576-7699-3|
|Publication Type:||Doctoral thesis|
|Full Text Status:||Public|
|Agris subject categories.:||F Plant production > F60 Plant physiology and biochemistry|
H Protection of plants and stored products > H20 Plant diseases
|Subjects:||(A) Swedish standard research categories 2011 > 1 Natural sciences > 104 Chemical Sciences > Analytical Chemistry|
(A) Swedish standard research categories 2011 > 1 Natural sciences > 104 Chemical Sciences > Organic Chemistry
|Agrovoc terms:||secondary metabolites, fraxinus excelsior, fungal diseases, chalara, pseudomonas, monographella nivalis, plant defense reactions, biological control, biological control agents, phytotoxins, steroids|
|Keywords:||Chalara fraxinea, Hymenoscyphus pseudoalbidus, secondary metabolites, steroids, biocontrol, Microdochium nivale, Pseudomonas|
|Department:||(NL, NJ) > Dept. of Chemistry (until 131231)|
|Deposited By:||Mr Pierre F. Andersson|
|Deposited On:||24 Aug 2012 08:57|
|Metadata Last Modified:||02 Dec 2014 10:51|
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