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Epidemiology and molecular tracing of bovine coronavirus and bovine respiratory syncytial virus infections in cattle herds

Bidokhti, Mehdi R. M. (2013). Epidemiology and molecular tracing of bovine coronavirus and bovine respiratory syncytial virus infections in cattle herds. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2013:34
ISBN 978-91-576-7807-2
[Doctoral thesis]

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Abstract

Bovine coronavirus (BCoV) and bovine respiratory syncytial virus (BRSV) are highly contagious pathogens that cause respiratory disease in cattle worldwide. BCoV is also associated with enteric disease in cattle of all ages. The aim of this thesis was to gain serological and molecular knowledge of BCoV and BRSV infections to help establish efficient future control in Sweden.

In the first study, the prevalence of antibodies to BCoV and BRSV infections was studied in 20 conventional and 20 organic dairy herds in south-east Sweden. On two occasions, with a 1-year interval, 699 serum samples from 624 cows were tested by ELISA. The antibody prevalence to BCoV and BRSV was high (> 80%) at both sampling times. Conventional herds had a significantly higher seroprevalence than the organic herds (P < 0.01). There was a significant association (p < 0.001) of antibody prevalence and age of the cow, where titres were higher in older individuals. The findings in this study suggest that organic farm management routines may be effective in reducing the seroprevalence of these viruses.

The second study was conducted on the molecular tracing of BCoV and BRSV throughout Sweden and also evolution of Betacoronavirus1. To investigate the molecular epidemiology of BCoV, the spike (S) gene of 27 PCR- positive samples from 2005 to 2009 were sequenced from 25 cattle herds. For BRSV, the glycoprotein (G) gene of PCR- positive samples during four years (2007 to 2011) were sequenced from 30 cattle herds. Sequence analysis revealed a high degree of identity among Swedish strains (> 97.7% for BCoV, > 94.5% for BRSV) regardless of clinical signs or animal age. Circulation of BCoV and BRSV between herds was found to occur throughout the year most often during the winter period. Identical sequences found in herds sampled within a few months' time suggested that these herds were part of a common transmission chain. Evolution analysis of the S gene of Betacoronavirus1 obtained since 1965 implies that BCoV strains are evolving genetically close to their human and canine counterparts. This study suggests that molecular analysis of strains can be a useful tool to support or rule out suspected transmission routes. Such knowledge is essential for the control of the spread of BCoV and BRSV between herds, regions and even countries.

Authors/Creators:Bidokhti, Mehdi R. M.
Title:Epidemiology and molecular tracing of bovine coronavirus and bovine respiratory syncytial virus infections in cattle herds
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :May 2013
Volume:2013:34
Number of Pages:73
Papers/manuscripts:
NumberReferences
I.Bidokhti, M.R., Tråvén, M., Fall, N., Emanuelson, U. & Alenius, S. (2009). Reduced likelihood of bovine coronavirus and bovine respiratory syncytial virus infection on organic compared to conventional dairy farms. Veterinary Journal 182(3), 436-40.
II.Bidokhti, M.R., Tråvén, M., Ohlson, A., Baule, C., Hakhverdyan, M., Belák, S., Liu, L. & Alenius, S. (2012). Tracing the transmission of bovine coronavirus infections in cattle herds based on S gene diversity. Veterinary Journal 193(2), 386-90.
III.Bidokhti, M.R., Tråvén, M., Ohlson, A., Zarnegar, B., Baule, C., Belák, S., Alenius, S. & Liu, L. (2012). Phylogenetic analysis of bovine respiratory syncytial viruses from recent outbreaks in feedlot and dairy cattle herds. Archives of Virology 157(4), 601-7.
IV.Bidokhti, M.R., Tråvén, M., Krishna N. K., Munir, M., Belák, S., Alenius, S., Cortey, M. Evolutionary Dynamics of Bovine Coronaviruses: Natural Selection Pattern of the Spike Gene Implies Adaptive Evolution of the Strains. (manuscript).
Place of Publication:Uppsala
Publisher:Dept. of Clinical Sciences, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-7807-2
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L73 Animal diseases
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Medical Bioscience
Agrovoc terms:coronavirus, bovine respiratory syncytial virus, bovine, disease transmission, epidemiology, dairy herds, organic husbandry, molecular genetics
Keywords:bovine coronavirus, spike gene, bovine respiratory syncytial virus, glycoprotein gene, epidemiology, organic, cattle herd, risk factors, molecular tracing, evolution
URN:NBN:urn:nbn:se:slu:epsilon-e-1344
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1344
ID Code:10280
Department:(VH) > Dept. of Clinical Sciences
Deposited By: Mehdi Bidokhti
Deposited On:24 Apr 2013 12:27
Metadata Last Modified:02 Dec 2014 10:59

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