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Renal scintigraphy in dogs

evaluation of glomerular filtration rate measurement by 99mTc-DTPA renogram

Kampa, Naruepon (2006). Renal scintigraphy in dogs. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2006:9
ISBN 91-576-7058-7
[Doctoral thesis]

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Renal scintigraphy has been used widely to measure the individual kidney glomerular filtration rate (IKGFR). In dogs, the estimated GFR is calculated by a regression equation relating the percent of the injected dose of radiopharmaceutical, technetium-99m DTPA (dietylene-triaminepentaacetic acid), taken up by the kidneys to the known GFR by standard (plasma) clearance normalized to bodyweight (BW). Uptake is calculated by the integral (area under the curve) of time activity curve (TAC) of the renogram, which is corrected for attenuation of radiation in the body from kidneys to body surface of the back and background activity. A more physiologically correct method is to normalize GFR to plasma volume (GFR/PV), which requires a region of interest (ROI) of the left ventricle (LV). The first aim of the thesis was to determine the variations within the standard method and minimize them to improve the accuracy of the methods. Physiological variability of GFR within and among dogs on different days was also studied. The integral method was more stable than a slope method and recommended to used for calculating IKGFR/BW. The best methods for measuring were: semi-automatic drawing of kidney ROI with 20% threshold, a perirenal background ROI at one pixel wide and one pixel out from kidney ROI, a threshold color scale rather than continuous for measuring kidney depth to calculate attenuation, and a time interval between 30 – 120 seconds from the start of the TAC. Measurement variation was also caused by significant observer variability, which indicates that methods must be standardized and the same person should measure all compared results. Physiologic day-to-day variability of GFR in normal dogs was mostly found between rather than within dogs, and accounted for most of the variability. GFR/PV was not affected by LV ROI sizes. Subtracting extravascular activity from the LV ROI did not improve precision, but increased variability due to different LV ROI sizes and time intervals chosen for LV plot. Manual LV ROI, without extravascular subtraction and a time interval for LV input between 1 and 4 minutes are recommended.

Authors/Creators:Kampa, Naruepon
Title:Renal scintigraphy in dogs
Subtitle:evaluation of glomerular filtration rate measurement by 99mTc-DTPA renogram
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :January 2006
Number of Pages:54
I.Kampa, N., Wennstrom, U., Lord, P., Twardock, R., Maripuu, E., Eksell, P. & Fredriksson, S.O. 2002. Effect of region of interest selection and uptake measurement on glomerular filtration rate measured by 99mTc-DTPA scintigraphy in dogs. Veterinary Radiology and Ultrasound 43, 383-391.
II.Kampa, N., Bostrom, I., Lord, P., Wennstrom, U., Ohagen, P. & Maripuu, E. 2003. Day-to-day variability in glomerular filtration rate in normal dogs by scintigraphic technique. Journal of Veterinary Medicine Series A, Physiology, Pathology, Clinical Medicine 50, 37-41.
III.Kampa, N., Lord, P. & Maripuu, E. 2006. Effect of observer variability on glomerular filtration rate measurement by renal scintigraphy in dogs. Veterinary Radiology and Ultrasound 47, 212-221.
IV.Kampa, N., Lord, P., Maripuu, E. & Hoppe, A. 2006. Glomerular filtration rate normalized to plasma volume by a scintigraphic method in dogs: effects of measurements of plasma activity input. Manuscript.
Place of Publication:Uppsala
Publisher:Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences
Associated Programs and Other Stakeholders:SLU - Research Areas for the Future > Future Animal Health and Welfare (until Jan 2017)
ISBN for printed version:91-576-7058-7
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L73 Animal diseases
L Animal production > L70 Veterinary science and hygiene - General aspects
Subjects:Not in use, please see Agris categories
Agrovoc terms:dogs, animal diseases, kidney diseases, blood volume, scintigraphy, diagnosis, analytical methods, veterinary medicine
Keywords:GFR, renal scintigraphy, dogs, 99mTc-DTPA, day-to-day variability, observer variability, plasma volume
Permanent URL:
ID Code:1030
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: Naruepon Kampa
Deposited On:31 Jan 2006 00:00
Metadata Last Modified:02 Dec 2014 10:09

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