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The putative endoglucanase PcGH61D from phanerochaete chrysosporium is a metal-dependent oxidative enzyme that cleaves cellulose

Sandgren, Mats and Wu, Miao and Ishida, Takuya (2011). The putative endoglucanase PcGH61D from phanerochaete chrysosporium is a metal-dependent oxidative enzyme that cleaves cellulose. PloS one. 6:11, 1-11
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Official URL: http://dx.doi.org/10.1371/journal.pone.0027807

Abstract

Many fungi growing on plant biomass produce proteins currently classified as glycoside hydrolase family 61 (GH61), some of which are known to act synergistically with cellulases. In this study we show that PcGH61D, the gene product of an open reading frame in the genome of Phanerochaete chrysosporium, is an enzyme that cleaves cellulose using a metal-dependent oxidative mechanism that leads to generation of aldonic acids. The activity of this enzyme and its beneficial effect on the efficiency of classical cellulases are stimulated by the presence of electron donors. Experiments with reduced cellulose confirmed the oxidative nature of the reaction catalyzed by PcGH61D and indicated that the enzyme may be capable of penetrating into the substrate. Considering the abundance of GH61-encoding genes in fungi and genes encoding their functional bacterial homologues currently classified as carbohydrate binding modules family 33 (CBM33), this enzyme activity is likely to turn out as a major determinant of microbial biomass-degrading efficiency.

Authors/Creators:Sandgren, Mats and Wu, Miao and Ishida, Takuya
Title:The putative endoglucanase PcGH61D from phanerochaete chrysosporium is a metal-dependent oxidative enzyme that cleaves cellulose
Series/Journal:PloS one (1932-6203)
Year of publishing :2011
Volume:6
Number:11
Page range:1-11
Number of Pages:11
Publisher:Public Library of Science
ISSN:1932-6203
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:X Agricola extesions > X30 Life sciences
X Agricola extesions > X50 Chemistry
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
Keywords:Oxidative enzyme, Putative Endoglucanase PcGH61D, fungi , GH61-encoding genes, enzyme activity, microbial biomass-degrading efficiency
URN:NBN:urn:nbn:se:slu:epsilon-e-1495
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1495
Additional ID:
Type of IDID
Web of Science (WoS)000298162000045
DOI10.1371/journal.pone.0027807
ID Code:10331
Department:(NL, NJ) > Dept. of Molecular Biology (until 131231)
Deposited By: SLUpub Connector
Deposited On:17 Jun 2013 09:18
Metadata Last Modified:24 Jan 2015 01:50

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