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Studies of plant interactions with other organisms to understand Bacillus mediated stress management

Johansson, Anna (2013). Studies of plant interactions with other organisms to understand Bacillus mediated stress management. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2013:42
ISBN 978-91-576-7823-2
[Doctoral thesis]

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Abstract

The naturally evolved plant defense system is not always effective, due to adaptations
among the attackers. Crop protective chemicals have many negative effects on the
environment. Ecosystem services, like beneficial microorganisms, are of great interest
for plant stress management in sustainable crop production. In this study, the
rhizobacterium Bacillus amyloliquefaciens, known to protect oilseed rape (Brassica
napus) to fungal diseases, was investigated. The aims were to test whether stains of
Bacillus could protect oilseed rape against insect herbivores, and to find out more about
factors involved in plant defense and Bacillus mediated stress protection.

Depending on the mode of treatment B. amyloliquefaciens were able to protect oilseed
rape plants against feeding by the generalist herbivore Spodoptera littoralis. Analysis
of transcripts and hormones implied involvement of JA signaling in Bacillus interaction
with oilseed rape, and metabolomic fingerprinting indicated special responses to the
Bacillus treatment. Real-time PCR (qPCR) assays were developed for the closely
related B. amyloliquefaciens strains UCMB5033, UCMB5036 and UCMB5113. Using
this, we revealed that mainly roots are colonized, and we saw a genotype dependence of
colonization and growth promotion efficiency on two oilseed rape cultivars. A test with
feeding by one generalist and one specialist herbivore on gene silenced (virus-induced)
and mutant Arabidopsis thaliana plants, revealed a complex role of the MD2-related
lipid recognition (ML3) gene in defense signaling, affecting both jasmonic acid (JA)
and salicylic acid (SA) associated responses. The plant protective ability of Bacillus
was investigated using A. thaliana Col-0 and An-1, and differential disease suppression
was found against a broad spectrum of pathogens but no mediated protection against a
specialist herbivore. Analyses of defense genes, hormone levels and mutants indicated
that UCMB5113 was capable of activating both SA and JA defense, dependent on
NPR1. Overall, the studies carried out revealed some mechanism of Bacillus mediated
priming of plant defense, involving resource allocation as part of a less costly defense
strategy.

Authors/Creators:Johansson, Anna
Title:Studies of plant interactions with other organisms to understand Bacillus mediated stress management
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :2013
Volume:2013:42
Number of Pages:63
Papers/manuscripts:
NumberReferences
I.Ingela Fridborg, Anna Johansson, Johanna Lagensjö, Natthanon Leelarasamee, Kristýna Floková, Danuše Tarkowska, Johan Meijer, and Sarosh Bejai (2013). ML3: a novel regulator of herbivory-induced responses in Arabidopsis thaliana. J. Exp. Bot. 64(4), 935-948.
II.Anna H. Johansson, Sarosh Bejai, Kristýna Floková, Danuše Tarkowská and Johan Meijer. Use of biotic and chemical agents for priming of oilseed rape (Brassica napus) defence against generalist insect herbivores. (manuscript).
III.Anna H. Johansson, Sarosh Bejai, Adnan Niazi, Shahid Manzoor, Erik Bongcam-Rudloff and Johan Meijer. Establishment of strain specific quantitative PCR assays for closely related Bacillus amyloliquefaciens biocontrol agents and studies of plant colonisation. (manuscript). IV Jesper Danielsson, Anna H. Johansson, Sarosh Bejai and Johan Meijer
IV.Jesper Danielsson, Anna H. Johansson, Sarosh Bejai and Johan Meijer. Bacillus mediated disease suppression in Arabidopsis thaliana. (manuscript).
V.Natthanon Leelarasamee, Sarosh Bejai, Anna Johansson, Kunling Chen, Shashidar Asari, Jonas Kjellin, Danuše Tarkowska and Johan Meijer. Multifaceted role of Bacillus amyloliquefaciens in priming of Arabidopsis thaliana defense responses against biotic stress (manuscript)
Place of Publication:Uppsala
Publisher:Institutionen för växtbiologi och skogsgenetik, Sveriges lantbruksuniversitet
ISBN for printed version:978-91-576-7823-2
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:H Protection of plants and stored products > H10 Pests of plants
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Agrovoc terms:brassica napus, oil crops, arabidopsis thaliana, plant models, bacillus, plant protection, plant defense reactions, stress, spodoptera littoralis
Keywords:Bacillus amyloliquefaciens, Oilseed rape, Arabidopsis, ISR
URN:NBN:urn:nbn:se:slu:epsilon-e-1444
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1444
ID Code:10412
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: Anna Helena Johansson
Deposited On:08 May 2013 11:44
Metadata Last Modified:14 Dec 2014 11:54

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