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Lipids in seeds of oat (Avena spp.), a potential oil crop

content, quality, metabolism, and possibilities for improvement

Leonova, Svetlana (2013). Lipids in seeds of oat (Avena spp.), a potential oil crop. Diss. (sammanfattning/summary) Alnarp : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2013:49
ISBN 978-91-576-7837-9
[Doctoral thesis]

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Abstract

New oil crops with high yield and oil content are urgently needed. Oat is the only cereal that accumulates substantial amount of lipids in the endosperm. This gives it potential as an oil crop, which could address both nutritional and environmental concerns of modern society. To develop oat with increased oil content, thorough investigations on its lipid metabolism and tools for genetic manipulations are needed.

Analyses of lipids in seeds of wild and cultivated oat species revealed variation in wild oat accessions in both oil content and quality. This variation should allow for development of new oat varieties for diverse applications. However, to develop oat as an oil crop, a level of 20% of the seed dry weight as oil is required and the range of oil content in the studied accessions was 4-10%. Another feature which showed almost no deviation was the amount of omega 3 fatty acid, -linolenic acid (0.6-2.1%). To develop oat varieties with ultra-high seed oil and increased content of omega 3 fatty acids, a biotechnological approach is preferred to conventional breeding methods.

Lipids in oat endosperm have been reported to exist, not enclosed in oil bodies, but as non-structured oil smear. In my study, I also observed oil smears, a phenomenon probably correlated with the reduced amount of oil body associated proteins in the endosperm, as revealed by microscopic and staining methods. This was supported experimentally by SDS-PAGE separation of oil-body proteins and immunoblotting and immunolocalization with antibodies against a 16 kD oil body protein.

Biochemical studies on lipid mobilization during oat seed germination demonstrated efficient utilization of oil reserves from the starchy endosperm; these results were corroborated by microscopy. An oat cultivar which is capable of accumulating high amounts of oil in the endosperm was shown to be also efficient in utilizing these reserves upon germination.

Agrobacterium-mediated transformation is one of the methods for modification of oat oil content and/or its quality. The results of the analyses aimed at development of an Agrobacterium-mediated oat transformation protocol are presented in this thesis.

Authors/Creators:Leonova, Svetlana
Title:Lipids in seeds of oat (Avena spp.), a potential oil crop
Subtitle:content, quality, metabolism, and possibilities for improvement
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :2013
Volume:2013:49
Number of Pages:60
Papers/manuscripts:
NumberReferences
I.Leonova, S., Shelenga, T., Hamberg, M., Konarev, A. V., Loskutov, I., & Carlsson, A. S. 2008, Analysis of oil composition in cultivars and wild species of oat (Avena sp.), Journal of Agricultural and Food Chemistry, 56(17):7983-7991.
II.Heneen, W. K., Karlsson, G., Brismar, K., Gummeson, P. O., Marttila, S., Leonova, S., Carlsson, A. S., Bafor, M., Banas, A., Mattsson, B., Debski, H., & Stymne, S. 2008, Fusion of oil bodies in endosperm of oat grains, Planta, 228(4):589-599.
III.Leonova, S., Grimberg, A., Marttila, S., Stymne, S., & Carlsson, A. S. 2010, Mobilization of lipid reserves during germination of oat (Avena sativa L.), a cereal rich in endosperm oil, Journal of Experimental Botany, 61(11):3089-3099.
IV.Leonova S., Carlsson A. S., Zhu L. Optimization of regeneration and transformation protocols for oat (Manuscript)
Place of Publication:Alnarp
Publisher:Institutionen för växtförädling, Sveriges lantbruksuniversitet
ISBN for printed version:978-91-576-7837-9
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
F Plant production > F60 Plant physiology and biochemistry
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
Agrovoc terms:avena, oats, cereal crops, oil crops, lipids, lipid content, fatty acids, proximate composition, endosperm, germination, agrobacterium
Keywords:oat, Avena, lipids, oil content, fatty acid composition, Agrobacterium-mediated transformation, tissue culture, regeneration, germination, cereal
URN:NBN:urn:nbn:se:slu:epsilon-e-1449
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1449
ID Code:10427
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
External funders:FORMAS and Royal Swedish Academy of Agriculture and Forestry and Swedish Institute
Deposited By: Unnamed user with email svetlana.leonova@slu.se
Deposited On:13 May 2013 07:57
Metadata Last Modified:13 Dec 2014 23:27

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