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Bioaugmentation for reduction of diffuse pesticide contamination

a bioprophylactic concept

Önneby, Karin (2013). Bioaugmentation for reduction of diffuse pesticide contamination. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2013:41
ISBN 978-91-576-7821-8
[Doctoral thesis]

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Abstract

Pesticides and their residues frequently contaminate surface waters and groundwater so consequently there is a great need to identify methods and practices that reduce such contamination. This thesis examined the potential of a 'bioprophylactic' concept based on the hypothesis that diffuse contamination after application in the field can be significantly reduced if pesticides are degraded as rapidly as possible after the intended effect has been achieved. This involves adding pesticide degraders together with the pesticide at the time of application. Such enhanced degradation is particularly important for pesticides used on non-agricultural soils with low degradation and high leaching potential (e.g. roadsides, railway embankments, paths, farmyards, urban land). The herbicide 4-chloro-2-methylphenoxyacetic acid (MCPA) was used as a model herbicide in this thesis, together with its degrader Sphingobium sp. T51. Soil inoculation with Sphingobium sp. T51 in microcosms significantly enhanced the degradation of MCPA compared with an uninoculated soil, without losing the herbicidal effect. In sand, 105 cells g-1 dry weight of soil reduced the MCPA concentration by 79-86% within one day, while only 0-31% reduction was observed in the uninoculated control after three days. However, Sphingobium sp. T51 needs to be formulated to retain its viability and degradation activity during prolonged storage and also for protection from the harsh environmental stresses associated with its application and functioning in the field. Both freeze drying and fluidised bed drying of Sphingobium sp. T51 resulted in high initial cell survival rates, of 67-85%. The storage stability of formulated Sphingobium sp. T51 was dependent on the formulation excipient/carrier used, storage temperature and atmospheric conditions. Sphingobium sp. T51 demonstrated approximately 50% survival in a freeze-dried sucrose formulation after six months of storage under partial vacuum at 25 °C. A fluidised bed-dried formulation with cottonseed flour as carrier resulted in the highest MCPA degradation in microcosms, but the storage stability was insufficient. Formulated and long-term stored Sphingobium sp. T51 reduced pesticide leaching by 85-94% of the total amount added in sand columns. Future research needs regarding practical implementation of the concept are discussed.

Authors/Creators:Önneby, Karin
Title:Bioaugmentation for reduction of diffuse pesticide contamination
Subtitle:a bioprophylactic concept
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :May 2013
Volume:2013:41
Number of Pages:75
Papers/manuscripts:
NumberReferences
I.Cederlund, H., Börjesson, E., Önneby, K. & Stenström, J (2007). Metabolic and cometabolic degradation of herbicides in the fine material of railway ballast. Soil Biology & Biochemistry 39(2), 473-484.
II.Önneby K., Jonsson A. & Stenström J. (2010). A new concept for reduction of diffuse contamination by simultaneous application of pesticide and pesticide-degrading microorganisms. Biodegradation 21 (1), 21-29.
III.Önneby K., Pizzul L., Bjerketorp J., Mahlin D., Håkansson S. & Wessman P (2013). Effects of di- and polysaccharide formulations and storage conditions on survival of freeze dried Sphingobium sp. World Journal of Microbiology and Biotechnology DOI: 10.1007/s11274-013-1303-7. In press.
IV.Önneby K., Håkansson S., Pizzul L. & Stenström J. Reduced leaching of the herbicide MCPA after bioaugmentation with a formulated and stored Sphingobium sp. Submitted.
Place of Publication:Uppsala
Publisher:Dept. of Microbiology, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-7821-8
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:H Protection of plants and stored products > H60 Weeds and weed control
P Natural resources > P30 Soil science and management
P Natural resources > P34 Soil biology
T Pollution > T01 Pollution
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 105 Earth and Related Environmental Sciences > Environmental Sciences (social aspects to be 507)
(A) Swedish standard research categories 2011 > 2 Engineering and Technology > 208 Environmental Biotechnology > Bioremediation
(A) Swedish standard research categories 2011 > 2 Engineering and Technology > 209 Industrial Biotechnology > Other Industrial Biotechnology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Soil Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 405 Other Agricultural Sciences > Environmental Sciences related to Agriculture and Land-use
Agrovoc terms:pesticides, nonpoint pollution, contamination, mcpa, herbicides, pollutants, biodegradation, bacteria, formulations
Keywords:bioaugmentation, pesticide degradation, formulation, leaching, diffuse contamination, Sphingobium, MCPA
URN:NBN:urn:nbn:se:slu:epsilon-e-1471
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1471
ID Code:10443
Department:(NL, NJ) > Dept. of Microbiology (until 161231)
External funders:FORMAS
Deposited By: Karin Önneby
Deposited On:21 May 2013 11:50
Metadata Last Modified:14 Dec 2014 19:58

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