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Yersinia enterocolitica in sheep - a high frequency of biotype 1A

Söderqvist, Karin and Boqvist, Sofia and Vågsholm, Ivar (2012). Yersinia enterocolitica in sheep - a high frequency of biotype 1A. Acta veterinaria Scandinavica. 54:39, 1-7
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Official URL: http://dx.doi.org/10.1186/1751-0147-54-39

Abstract

BACKGROUND:
Pigs are regarded as the main reservoir for human pathogenic
Yersinia enterocolitica, which is dominated by bioserotype 4/O:3. Other animals, including sheep, have occasionally been reported as carriers of pathogenic strains of
Y. enterocolitica. To our knowledge, this is the first study performed in the Nordic countries in which the presence of Y. enterocolitica in sheep is investigated.

METHODS:
Tonsils and faecal samples collected from sheep slaughtered on the island Gotland (Sweden) from September 2010 through January 2011 were analysed for presence of Y. enterocolitica. In an attempt to maximize recovery, several cultural strategies were applied. Various non-selective media were used and different temperatures and durations of the enrichment were applied before subculturing on Cefsulodin Irgasan Novobiocin (CIN) agar. Presumptive
Y. enterocolitica colonies were subjected to urease, API 20E and agglutination test. Yersinia enterocolitica
isolates were biotyped, serotyped, and tested for pathogenicity using a TaqMan PCR directed towards the
ail-gene that is associated with human pathogenic strains of
Y. enterocolitica.

RESULTS:
The samples collected from 99 sheep yielded 567 presumptive
Y. enterocolitica colonies. Eighty urease positive isolates, from 35 sheep, were identified as Y. enterocolitica by API 20E. Thirty-four of 35 further subtyped Y. enterocolitica isolates, all from faecal samples, belonged to biotype 1A serotype O:5, O:6. O:13,7 and O:10. One strain was Yersinia mollaretii serotype O:62. No human pathogenic strains of Y. enterocolitica were found in the investigated sheep. Other species identified were
Y. kristensenii (n=4), Y. frederiksenii/intermedia (n =3),
Providencia rettgeri (n= 2), Serratia marcescens (n =1) and
Raoultella ornithinolytica (n=1).

CONCLUSIONS:
This study does not support the hypothesis that sheep play an important role in transmission of the known human pathogenic Y. enterocolitica in the studied geographical region. However, because there are studies indicating that some strains of Y. enterocolitica biotype 1A may cause disease in humans, the relative importance of sheep as carriers of human pathogenic strains of Y. enterocolitica
remains unclear. Tonsils do not appear to be favourable sites for Y. enterocolitica biotype 1A in sheep.

Authors/Creators:Söderqvist, Karin and Boqvist, Sofia and Vågsholm, Ivar
Title:Yersinia enterocolitica in sheep - a high frequency of biotype 1A
Series/Journal:Acta veterinaria Scandinavica (0044-605X)
Year of publishing :2012
Volume:54
Number:39
Page range:1-7
Number of Pages:7
Publisher:BioMed Central
ISSN:0044-605X
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:L Animal production > L50 Animal physiology and biochemistry
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology
Keywords:Food safety , Sheep, yersinia enterocolitica, Microbiology, Sweden
URN:NBN:urn:nbn:se:slu:epsilon-e-1499
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1499
Additional ID:
Type of IDID
Web of Science (WoS)000308301200001
DOI10.1186/1751-0147-54-39
ID Code:10446
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: SLUpub Connector
Deposited On:17 Jun 2013 10:52
Metadata Last Modified:22 Feb 2016 05:57

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