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Seed colour loci, homoeology and linkage groups of the C genome chromosomes revealed in Brassica rapa - B. oleracea monosomic alien addition lines

Heneen, Waheeb and Geleta, Mulatu Dida and Brismar, Kerstin and Xiong, Zhiyong and Pires, J.C. and Hasterok, Robert and Stoute, Andrew I and Scott, Roderick J and King, Graham J and Kurup, Smita (2012). Seed colour loci, homoeology and linkage groups of the C genome chromosomes revealed in Brassica rapa - B. oleracea monosomic alien addition lines. Annals of botany. 109:7, 1227-1242
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Official URL: http://dx.doi.org/10.1093/aob/mcs052

Abstract

Background and Aims Brassica rapa and B. oleracea are the progenitors of oilseed rape B. napus. The addition of each chromosome of B. oleracea to the chromosome complement of B. rapa results in a series of monosomic alien addition lines (MAALs). Analysis of MAALs determines which B. oleracea chromosomes carry genes controlling specific phenotypic traits, such as seed colour. Yellow-seeded oilseed rape is a desirable breeding goal both for food and livestock feed end-uses that relate to oil, protein and fibre contents. The aims of this study included developing a missing MAAL to complement an available series, for studies on seed colour control, chromosome homoeology and assignment of linkage groups to B. oleracea chromosomes. † Methods A new batch of B. rapa–B. oleracea aneuploids was produced to generate the missing MAAL. Seed colour and other plant morphological features relevant to differentiation of MAALs were recorded. For chromosome characterization, Snow’s carmine, fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) were used. † Key Results The final MAAL was developed. Morphological traits that differentiated the MAALs comprised cotyledon number, leaf morphology, flower colour and seed colour. Seed colour was controlled by major genes on two B. oleracea chromosomes and minor genes on five other chromosomes of this species. Homoeologous pairing was largely between chromosomes with similar centromeric positions. FISH, GISH and a parallel microsatellite marker analysis defined the chromosomes in terms of their linkage groups. †Conclusions A complete set of MAALs is now available for genetic, genomic, evolutionary and breeding perspectives. Defining chromosomes that carry specific genes, physical localization of DNA markers and access to established genetic linkage maps contribute to the integration of these approaches, manifested in the confirmed correspondence of linkage groups with specific chromosomes. Applications include marker-assisted selection and breeding for yellow seeds.

Authors/Creators:Heneen, Waheeb and Geleta, Mulatu Dida and Brismar, Kerstin and Xiong, Zhiyong and Pires, J.C. and Hasterok, Robert and Stoute, Andrew I and Scott, Roderick J and King, Graham J and Kurup, Smita
Title:Seed colour loci, homoeology and linkage groups of the C genome chromosomes revealed in Brassica rapa - B. oleracea monosomic alien addition lines
Series/Journal:Annals of botany (1095-8290)
Year of publishing :2012
Volume:109
Number:7
Page range:1227-1242
Number of Pages:16
Publisher:Oxford University Press, for Annals of Botany Company
ISSN:1095-8290
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Accepted version
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402)
Keywords:Brassica rapa var. trilocularis, B. oleracea var. alboglabra, MAALs, , 9 characterization of C chromosomes,, plant morphology, seed colour control, FISH, 10 GISH, chromosome homoeology, chromosome structural changes, linkage groups, 11 crop plant breeding
URN:NBN:urn:nbn:se:slu:epsilon-e-1725
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1725
Additional ID:
Type of IDID
Web of Science (WoS)WOS:000304532300003
DOI10.1093/aob/mcs052
ID Code:10556
Department:(LTJ, LTV) > Department of Plant Protection Biology
(LTJ, LTV) > Plant Breeding and Biotechnology (until 121231)
Deposited By: SLUpub Connector
Deposited On:12 Dec 2013 13:29
Metadata Last Modified:27 Feb 2015 03:44

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