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cDNA cloning and expression analysis of pattern recognition proteins from the Chinese Oak Silkmoth, Antheraea pernyi

Li, Fengjuan and Terenius, Olle and Yuan, Li and Fang, Suyun and Li, Wenli (2012). cDNA cloning and expression analysis of pattern recognition proteins from the Chinese Oak Silkmoth, Antheraea pernyi. Insects. 3:4, 1093-1104
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Official URL: http://dx.doi.org/10.3390/insects3041093

Abstract

Pattern recognition receptors play an important role in insect immune defense. We cloned the β-1,3-glucan recognition protein, lectin-5 and C-type lectin 1 genes of Antheraea pernyi and examined the expression profiles of immune-stimulated pupae. After infection with Bacillus subtilis, Escherichia coli, Antheraea pernyi nuclear polyhedrosis virus (ApNPV) and Saccharomyces cerevisiae, respectively, the pupae showed different gene expression levels in the different tissues examined (midgut, fatbody, epidermis, testis, and hemocytes). ApβGRP and Aplectin-5 was induced by all the microorganisms, and mainly in epidermis and hemocytes, but not in testis; Aplectin-5 was also expressed in fatbody. Ap C-type lectin 1 was, on the contrary, highly expressed in testis and also in fatbody, but not in hemocytes. Unlike ApβGRP and Aplectin-5, Ap C-type lectin 1 was not induced by Gram-positive bacteria. The results suggest that the cloned lectins may have different functions in different tissues of A. pernyi.

Authors/Creators:Li, Fengjuan and Terenius, Olle and Yuan, Li and Fang, Suyun and Li, Wenli
Title:cDNA cloning and expression analysis of pattern recognition proteins from the Chinese Oak Silkmoth, Antheraea pernyi
Series/Journal:Insects (2075-4450)
Year of publishing :2012
Volume:3
Number:4
Page range:1093-1104
Number of Pages:12
Publisher:Molecular Diversity Preservation International (MDPI), Switzerland
ISSN:2075-4450
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:F Plant production > F60 Plant physiology and biochemistry
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Immunology (Immunology in the medical area to be 30110)
Keywords:insect immunity , Antheraea pernyi, betaGRP, lectin, gene expression
URN:NBN:urn:nbn:se:slu:epsilon-e-1529
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1529
Additional ID:
Type of IDID
DOI10.3390/insects3041093
ID Code:10590
Department:(NL, NJ) > Dept. of Ecology
(S) > Dept. of Ecology
Deposited By: SLUpub Connector
Deposited On:04 Jul 2013 14:43
Metadata Last Modified:23 Feb 2015 23:52

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