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Genetic improvement of oil quality in sesame (Sesamum indicum L.)

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Were, Beatrice Ang'iyo (2006). Genetic improvement of oil quality in sesame (Sesamum indicum L.). Diss. (sammanfattning/summary) Alnarp : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2006:12
ISBN 91-576-7061-7
[Doctoral thesis]

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Sesame (Sesamum indicum L.) seed is source of high quality edible oil for which the market niche can be expanded by developing cultivars with altered fatty acid composition through conventional breeding or genetic transformation. There is need to know the natural diversity in oil quality to facilitate breeding, while reliable regeneration and transformation protocols need to be developed for genetic engineering. The seed oil diversity in thirty sesame accessions from East Africa over three years, and the regeneration and floral transformation potential of selected accessions were studied. The effectiveness of a cloned sesame oleate desaturase was tested in Arabidopsis thaliana. Significant variation was observed among the accessions for the oil content and the quantity of individual fatty acids in each of the three years of field evaluation. There was positive correlation between oil content and stearic and oleic acid levels. Shoot regeneration was achieved from cotyledon explants, whereas hypocotyl explants only gave callus and roots. Some accessions were more amenable to regeneration than others. A high rate of sesame transformation using Agrobacterium tumefaciens carrying a binary vector containing the neomycin phosphotransferase (NPT) II gene for kanamycin resistance and the enhanced green fluorescent protein (EGFP) reporter gene was achieved through floral dipping, pollen infiltration and suspension drop. Pollen infiltration and suspension drop produced higher transformation frequencies than floral dip. Agrobacterium strains EHA 105 and GV3101 gave higher transformation than GV2260. The best sesame cultivars for transformation were Mtwara-2 and McBlack. A Δ12 oleate desaturase cDNA isolated from immature seeds had its function confirmed by its complementation of the fad2-2 mutant phenotype of A. thaliana. The cDNA was also expressed in transgenic A. thaliana lines that synthesize epoxy, hydroxy and acetylenic fatty acids, and shown to influence the accumulation of linoleic and the unusual fatty acids. The major contribution of this study is the development of novel regeneration and transformation techniques for sesame, which open new avenues for the genetic improvement of the crop.

Authors/Creators:Were, Beatrice Ang'iyo
Title:Genetic improvement of oil quality in sesame (Sesamum indicum L.)
Subtitle:assembling tools
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :March 2006
Number of Pages:40
ALLI. Were, B. A., Onkware, A. O., Gudu, S., Welander, M. & Carlsson, A. S. 2006. Seed oil content and fatty acid composition in East African sesame (Sesamum indicum L.) accessions evaluated over three years. Field crops research (In press). II. Were, B. A., Gudu, S., Onkware, A. O., Carlsson, A. S & Welander, M. 2006. In vitro regeneration of sesame (Sesamum indicum L.) from seedling cotyledon and hypocotyl explants. Plant cell tissue and organ culture (In press). III. Were, B. A., Carlsson, A. S., Welander, M., Onkware, A. O. and Gudu, S. 2006. Floral transformation of sesame (Sesamum indicum L.) using Agrobacterium tumefaciens. Plant cell reports (Submitted). IV. Were, B. A., Onkware, A. O., Gudu, S., Welander, M. & Carlsson, A. S. 2006. Expression of a Sesamum indicum Δ12 desaturase alters levels of unusual fatty acids in seed oil of transgenic Arabidopsis thaliana (Manuscript).
Place of Publication:Alnarp
ISBN for printed version:91-576-7061-7
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
Subjects:Not in use, please see Agris categories
Agrovoc terms:sesamum indicum, sesame oil, genetic transformation, fatty acids, quality, agrobacterium tumefaciens
Keywords:Sesamum indicum, oil quality, fatty acids, regeneration, genetic transformation, Agrobacterium, Δ12 oleate desaturase.
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ID Code:1061
Department:?? 4806 ??
Deposited By: Beatrice Ang'iyo Were
Deposited On:15 Mar 2006 00:00
Metadata Last Modified:02 Dec 2014 10:09

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