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Reduced expression of the SHORT-ROOT gene increases the rates of growth and development in hybrid poplar and arabidopsis

Wang, Jiehua and Gunnerås, Sara and Gaboreanu, Ioana and Tucker, M. and Zheng, Bo and Lesniewska, Joanna and Mellerowicz, Ewa and Laux, Thomas and Sandberg, Göran and Jones, Brian (2011). Reduced expression of the SHORT-ROOT gene increases the rates of growth and development in hybrid poplar and arabidopsis. PloS one. 6:12, 1-9
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Official URL: http://dx.doi.org/10.1371/journal.pone.0028878

Abstract

SHORT-ROOT (SHR) is a well characterized regulator of cell division and cell fate determination in the Arabidopsis primary root. However, much less is known about the functions of SHR in the aerial parts of the plant. In this work, we cloned SHR gene from Populus trichocarpa (PtSHR1) as an AtSHR ortholog and down-regulated its expression in hybrid poplar (Populus tremula6P. tremuloides Michx-clone T89) in order to determine its physiological functions in shoot development. Sharing a 90% similarity to AtSHR at amino acid level, PtSHR1 was able to complement the Arabidopsis shr mutant. Down regulation of PtSHR1 led to a strong enhancement of primary (height) and secondary (girth) growth rates in the transgenic poplars. A
similar approach in Arabidopsis showed a comparable accelerated growth and development phenotype. Our results suggest that the response to SHR could be dose-dependent and that a partial down-regulation of SHR could lead to enhanced meristem activity and a coordinated acceleration of plant growth in woody species. Therefore, SHR functions in plant growth and development as a regulator of cell division and meristem activity not only in the roots but also in the shoots.

Reducing SHR expression in transgenic poplar was shown to lead to significant increases in primary and secondary growth rates. Given the current interest in bioenergy crops, SHR has a broader role as a key regulator of whole plant growth and development and SHR suppression has considerable potential for accelerating biomass accumulation in a variety of species.

Authors/Creators:Wang, Jiehua and Gunnerås, Sara and Gaboreanu, Ioana and Tucker, M. and Zheng, Bo and Lesniewska, Joanna and Mellerowicz, Ewa and Laux, Thomas and Sandberg, Göran and Jones, Brian
Title:Reduced expression of the SHORT-ROOT gene increases the rates of growth and development in hybrid poplar and arabidopsis
Series/Journal:PloS one (1932-6203)
Year of publishing :2011
Volume:6
Number:12
Page range:1-9
Number of Pages:9
Publisher:Public Library of Science
ISSN:1932-6203
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
F Plant production > F62 Plant physiology - Growth and development
K Forestry > K10 Forestry production
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
Keywords:forest productivity, bioenergy, biomaterials, Short-root (SHR), Arabidopsis, bioenergy crops, plant growth, plant development, biomass accumulation
URN:NBN:urn:nbn:se:slu:epsilon-e-1591
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1591
Additional ID:
Type of IDID
Web of Science (WoS)000298369100117
DOI10.1371/journal.pone.0028878
ID Code:10714
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:21 Aug 2013 10:41
Metadata Last Modified:07 Dec 2014 16:17

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