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A Genome-Wide Association Study Identifies Genomic Regions for Virulence in the Non-Model Organism Heterobasidion annosum ss

Dalman, Kerstin and Himmelstrand, Kajsa and Olson, Åke and Lind, Mårten and Brandström Durling, Mikael and Stenlid, Jan (2013). A Genome-Wide Association Study Identifies Genomic Regions for Virulence in the Non-Model Organism Heterobasidion annosum ss. . 8:1, 1-10
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Abstract

The dense single nucleotide polymorphisms (SNP) panels needed for genome wide association (GWA) studies have hitherto
been expensive to establish and use on non-model organisms. To overcome this, we used a next generation sequencing
approach to both establish SNPs and to determine genotypes. We conducted a GWA study on a fungal species, analysing
the virulence of Heterobasidion annosum s.s., a necrotrophic pathogen, on its hosts Picea abies and Pinus sylvestris. From a
set of 33,018 single nucleotide polymorphisms (SNP) in 23 haploid isolates, twelve SNP markers distributed on seven contigs
were associated with virulence (P,0.0001). Four of the contigs harbour known virulence genes from other fungal
pathogens and the remaining three harbour novel candidate genes. Two contigs link closely to virulence regions
recognized previously by QTL mapping in the congeneric hybrid H. irregulare6H. occidentale. Our study demonstrates the
efficiency of GWA studies for dissecting important complex traits of small populations of non-model haploid organisms with
small genomes.

Authors/Creators:Dalman, Kerstin and Himmelstrand, Kajsa and Olson, Åke and Lind, Mårten and Brandström Durling, Mikael and Stenlid, Jan
Title:A Genome-Wide Association Study Identifies Genomic Regions for Virulence in the Non-Model Organism Heterobasidion annosum ss
Series/Journal:PLoS One
Year of publishing :2013
Volume:8
Number:1
Page range:1-10
Number of Pages:10
Publisher:Public Library of Science
ISSN:1932-6203
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402)
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
Keywords:genetics, forest science, Heterobasidion annosum s.s.,, Picea Abies, Pinus sylvestris, pathogens, fungae
URN:NBN:urn:nbn:se:slu:epsilon-e-1594
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1594
Additional ID:
Type of IDID
Web of Science (WoS)000313682700027
DOI10.1371/journal.pone.0053525
ID Code:10755
Department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
External funders:FORMAS and Swedish Foundation for Strategic Research
Deposited By: SLUpub Connector
Deposited On:22 Aug 2013 06:05
Metadata Last Modified:21 Feb 2015 12:27

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