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Research article - Peer-reviewed, 2012

Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species

Schwochow, Doreen; Serieys, Laurel E.K.; Wayne, Robert K.; Thalmann, Olaf

Abstract

Conclusion: By carefully choosing the appropriate RNA extraction method, whole blood can become a valuable source for high-throughput applications like expression arrays or transcriptome sequencing from natural populations. Additionally, candidate genes showing signs of selection could subsequently be genotyped in large population samples using whole blood as a source for RNA without harming individuals from rare or endangered species.

Keywords

Whole blood; RNA preservation; RNA extraction; DNA contamination; Transcriptome sequencing; Globin transcripts

Published in

BMC Biotechnology
2012, Volume: 12, pages: 1-11
Publisher: BIOMED CENTRAL LTD

    UKÄ Subject classification

    Microbiology
    Other Environmental Biotechnology

    Publication identifier

    DOI: https://doi.org/10.1186/1472-6750-12-33

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/45166