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Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species

Schwochow, Doreen and Serieys, Laurel E.K. and Wayne, Robert K. and Thalmann, Olaf (2012). Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species. BMC Biotechnology. 12, 1-11
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Abstract

Conclusion: By carefully choosing the appropriate RNA extraction method, whole blood can become a valuable source for high-throughput applications like expression arrays or transcriptome sequencing from natural populations. Additionally, candidate genes showing signs of selection could subsequently be genotyped in large population samples using whole blood as a source for RNA without harming individuals from rare or endangered species.

Authors/Creators:Schwochow, Doreen and Serieys, Laurel E.K. and Wayne, Robert K. and Thalmann, Olaf
Title:Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species
Series/Journal:BMC Biotechnology (1472-6750)
Year of publishing :2012
Volume:12
Page range:1-11
Number of Pages:12
Publisher:BioMed Central Ltd.
ISSN:1472-6750
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 2 Engineering and Technology > 208 Environmental Biotechnology > Other Envionmental Biotechnology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
Agrovoc terms:Blood sampling, Animal genetics, Wildlife
Keywords:Whole blood, RNA preservation, RNA extraction, DNA contamination, Transcriptome sequencing, Globin transcripts
URN:NBN:urn:nbn:se:slu:epsilon-e-1660
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1660
Additional ID:
Type of IDID
Web of Science (WoS)000306833900001
DOI10.1186/1472-6750-12-33
ID Code:10833
Department:(VH) > Dept. of Molecular Biosciences (until 070101)
Deposited By: SLUpub Connector
Deposited On:10 Oct 2013 06:42
Metadata Last Modified:02 Dec 2014 11:03

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