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Phyllosphere of organically grown strawberries

interactions between the resident microbiota, pathogens and introduced microbial agents

Sylla, Justine (2013). Phyllosphere of organically grown strawberries. Diss. (sammanfattning/summary) Alnarp : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2013:85
ISBN 978-91-576-7908-6
eISBN 978-91-576-7909-3
[Doctoral thesis]

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Abstract

The use of biological control agents (BCAs) is regarded as a promising measure to control important foliar strawberry diseases such as grey mould (Botrytis cinerea) and powdery mildew (Podosphaera aphanis) in the organic strawberry cultivation. However, the use of biological control agents (BCAs) in the phyllosphere is still challenging as this environment is very harsh and dynamic, in particular under field conditions. In this thesis, the simultaneous use of BCAs was studied for its potential to overcome the challenges biological control in the phyllosphere imposes and, thereby, to achieve more consistent efficacies against B. cinerea and P. aphanis.

In vitro tests revealed that inhibitory interactions can basically occur between two BCAs and that these are affected by nutritional factors. Leaf disc assays demonstrated that the simultaneous use of BCAs can result in improved suppression of P. aphanis, depending on the BCA constituents. Furthermore, several BCAs were applied as single or multiple strain treatments against B. cinerea in three years of field experiment and microbial interactions in the phyllosphere were investigated within these experiments.

The simultaneous use of BCAs did not result in consistent B. cinerea control under field conditions. In the field experiment 2010, none of the tested single or multiple BCA treatments reduced B. cinerea. In the field experiments 2011 and 2012 B. cinerea incidence was significantly suppressed by simultaneously applied BCAs as opposed to single BCA treatments. Efficient BCA treatments, however, differed in 2011 and 2012. Microbial analyses on leaves from field grown strawberries by means of plate counts and 454 pyrosequencing revealed that the culturable and the non-culturable resident leaf microbiota considerably varied between different years of experiment but also in dependence on the strawberry's development stage. Likewise, the interactions between the resident microbial communities and introduced BCAs varied as well, which have shown to be associated with inconsistent efficacies of the tested BCAs in 2011 and 2012. Also, microbial investigations revealed shifts in fungal communities after introducing fungal BCAs, which however can be regarded as neglible due to the overall considerable dynamics of the phyllosphere microbiota.

Authors/Creators:Sylla, Justine
Title:Phyllosphere of organically grown strawberries
Subtitle:interactions between the resident microbiota, pathogens and introduced microbial agents
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :12 November 2013
Volume:2013:85
Number of Pages:68
Papers/manuscripts:
NumberReferences
I.Justine Sylla, Beatrix W. Alsanius, Erika Krüger, Dorit Becker and Walter Wohanka (2013). In vitro compatibility of microbial agents for simultaneous application to control strawberry powdery mildew (Podosphaera aphanis). Crop Protection 51, 40-47.
II.Justine Sylla, Beatrix W. Alsanius, Erika Krüger, Annette Reineke, Stephan Strohmeier and Walter Wohanka (2013). Leaf microbiota of strawberries as affected by biological control agents. Phytopathology 103, 1001-1011.
III.Justine Sylla, Beatrix W. Alsanius, Erika Krüger, Annette Reineke, Monika Bischoff-Schaefer and Walter Wohanka (2013). Introduction of Aureobasidium pullulans to the phyllosphere of organically grown strawberries with focus on its establishment and interactions with the resident microbiome. Agronomy 3(4), 704-731.
IV.Justine Sylla, Beatrix W. Alsanius, Erika Krüger and Walter Wohanka. Control of Botrytis cinerea in organically grown strawberries by biological control agents applied as single or multiple strain treatments (manuscript).
Place of Publication:Alnarp
Publisher:Department of Biosystems and Technology, Swedish University of Agricultural Sciences
Associated Programs and Other Stakeholders:Z - SLU - Libray > Odla mera
ISBN for printed version:978-91-576-7908-6
ISBN for electronic version:978-91-576-7909-3
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:H Protection of plants and stored products > H20 Plant diseases
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 405 Other Agricultural Sciences > Environmental Sciences related to Agriculture and Land-use
Agrovoc terms:fragaria ananassa, strawberries, phyllosphere, pathogens, botrytis cinerea, moulds, podosphaera, powdery mildews, organic agriculture, biological control agents
Keywords:microbial communities, phyllosphere, biocontrol, strawberry
URN:NBN:urn:nbn:se:slu:epsilon-e-1700
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1700
ID Code:10895
Department:(LTJ, LTV) > Department of Biosystems and Technology (from 130101)
(VH) > Department of Biosystems and Technology (from 130101)
Deposited By: Justine Sylla
Deposited On:13 Nov 2013 08:08
Metadata Last Modified:22 Mar 2015 15:09

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