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Developmental phase transitions in Norway spruce

a molecular approach to identify regulatory mechanisms

Uddenberg, Daniel (2013). Developmental phase transitions in Norway spruce. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2013:94
ISBN 978-91-576-7926-0
eISBN 978-91-576-7927-7
[Doctoral thesis]

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Abstract

Plant development proceeds through distinct phases that are controlled by complex networks of regulatory genetic circuits and fine-tuned by environmental and endogenous cues. Many of these regulatory networks have been unraveled in annual and perennial angiosperms, while they remain predominantly unknown in ecologically and economically important gymnosperm species such as the conifers.

By assessing global gene expression profiles during early somatic embryo development in Norway spruce we identified transcripts potentially associated with the transition from the embryonal to the vegetative phase. A conifer transcript (PaHAP3A) homologous to LEAFY COTYLEDON1 (LEC1), a well-known master regulator of Arabidopsis embryogenesis, was characterized. PaHAP3A is active during early embryo development and is down-regulated during embryo maturation. Overexpression of PaHAP3A during embryo maturation causes differentiation of ectopic embryos. Together, our results support sub- and/or neofunctionalization between angiosperm and gymnosperm LEC1-type genes. Global inhibition of histone deacetylase (HDAC) action during embryo maturation alters the patterns of embryonic gene expression and arrests the maturation progression, while HDAC inhibition during germination retains the embryogenic potential.

In the naturally occurring, early cone-setting Norway spruce mutant acrocona, vegetative shoots typically display a transition from vegetative to female identity. By in situ hybridization assays, expression patterns of previously identified and novel MADS-box genes were characterized in female cones from wild-type and acrocona plants at distinct developmental stages. Furthermore, an RNA sequencing approach utilizing a population of inbred acrocona plants identified a MADS-box gene (DAL19) as a potential important factor for the initiation of the seed cone.

Taken together, this thesis presents novel insights into the regulatory networks that control important phases during the life cycle of Norway spruce, indicating both conservation and functional divergence between gymnosperms and angiosperms.

Authors/Creators:Uddenberg, Daniel
Title:Developmental phase transitions in Norway spruce
Subtitle:a molecular approach to identify regulatory mechanisms
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :20 December 2013
Volume:2013:94
Number of Pages:73
Papers/manuscripts:
NumberReferences
I.Vestman D., Larsson E., Uddenberg D., Cairney J., Clapham D., Sundberg E., von Arnold S. (2011). Important processes during differentiation and early development of somatic embryos of Norway spruce as revealed by changes in global gene expression. Tree Genetics and Genomes 7, 347- 362.
II.Uddenberg D., Valladares S., Abrahamsson M., Sundström J.F., Sundås- Larsson A., von Arnold S. (2011). Embryogenic potential and expression of embryogenesis-related genes in conifers are affected by treatment with a deacetylase inhibitor. Planta 234(3), 527-539.
III.Uddenberg D., Reimegård J., Clapham D., Almqvist C., von Arnold S., Emanuelsson O., Sundström J.F. (2013). Early cone setting in Picea abies acrocona is associated with increased expression of a MADS box transcription factor. Plant Physiology 161(2), 813-823.
IV.Carlsbecker A., Sundström J.F., Englund M., Uddenberg D., Izquierdo L., Kvarnheden A., Vergara-Silva F., Engström P. (2013) Molecular control of normal and acrocona mutant seed cone development in Norway spruce (Picea abies) and the evolution of conifer ovule-bearing organs. New Phytologist 200(1), 261-275.
Place of Publication:Uppsala
Publisher:Dept. of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-7926-0
ISBN for electronic version:978-91-576-7927-7
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
F Plant production > F62 Plant physiology - Growth and development
K Forestry > K01 Forestry - General aspects
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402)
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Developmental Biology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
Agrovoc terms:picea abies, plant embryos, plant developmental stages, somatic embryogenesis, gene expression, gene transcription
Keywords:conifers, embryogenic potential, HDAC inhibition, LEC1, MADS-box, phase transitions, Picea abies var. acrocona, somatic embryogenesis
URN:NBN:urn:nbn:se:slu:epsilon-e-1737
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1737
ID Code:10953
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: Daniel Uddenberg
Deposited On:20 Dec 2013 09:46
Metadata Last Modified:02 Dec 2014 11:04

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