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The toxicity of perfluoroalkyl acids in zebrafish (Danio rerio)

Ul Haq, Mazhar (2013). The toxicity of perfluoroalkyl acids in zebrafish (Danio rerio). Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2013:95
ISBN 978-91-576-7928-4
eISBN 978-91-576-7929-1
[Doctoral thesis]

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Perfluoroalkyl acids (PFAAs) are globally distributed synthetic chemicals. Their unique properties make them widely used, in industry and elsewhere. These are a group of compounds with varying carbon chain lengths and functional groups. The toxicity evaluation of PFAAs is important to better assess their organismal and environmental impact.

The developmental toxicity of a group of PFAAs was evaluated in zebrafish (Danio rerio), using the embryotoxicity and locomotor behavior endpoints. Structure related relationships were examined using endpoints based on lethal and sublethal effect. The EC50 based toxicity ranking of the PFAAs indicated that the toxicity pattern was governed by carbon chain length and attached functional group. The behavior analyses showed that the PFAAs have distinct patterns in terms of locomotor activity of larvae.

Radiolabeled 14C-PFOA was used to investigate the uptake, kinetics and distribution of the chemical in zebrafish. An uptake and elimination approach to experimentation was employed for kinetic assessment. A parallel equilibrium model bioconcentration strategy for 100-fold exposure range determined the internal body burden. Whole-body autoradiography and liquid scintillation techniques revealed the tissue distribution of PFOA. Remarkable levels of radioactivity were observed in target organs. The labeling of oocytes confirmed the maternal transfer of PFOA in zebrafish.

In another study the effects of PFOA on reproduction and sexual development in zebrafish were evaluated. Reproductive traits like spawning, fecundity and fertilization were not altered in a short term experiment. The period of sexual differentiation in zebrafish was examined to determine the effects of PFOA on development, growth and gonad maturation. The development and growth of the zebrafish were not affected, but a trend in decreased survival in maternally exposed embryos was observed. The expression of Vtg mRNA level in the liver of PFOA-exposed male fish was not induced. Similarly, an inhibitory trend of PPARα mRNA expression in the liver of the highest PFOA exposed zebrafish was observed.

The developmental toxicity ranking of PFAAs and kinetic assessment of PFOA with bioconcentration in zebrafish will provide an insight into risk assessment and replacement strategies of the compounds.

Authors/Creators:Ul Haq, Mazhar
Title:The toxicity of perfluoroalkyl acids in zebrafish (Danio rerio)
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :2013
Number of Pages:76
I.Ulhaq, M., Carlsson, G., Örn, S. & Norrgren, L. 2013. Comparison of developmental toxicity of seven perfluoroalkyl acids to zebrafish embryos. Environmental Toxicology and Pharmacology 36, 423-426.
II.Ulhaq, M., Örn, S., Carlsson, G., Morrison, D. A. & Norrgren, L. 2013. Locomotor behavior in zebrafish (Danio rerio) larvae exposed to perfluoroalkyl acids. Aquatic Toxicology 144-145C, 332-340.
III.Ulhaq, M., Örn, S., Sundström, M., Larsson, P., Gabrielsson, J., Bergman, Å. & Norrgren, L. Tissue uptake, distribution and elimination of 14CPFOA in zebrafish (Danio rerio). (Submitted)
IV.Ulhaq, M., Örn, S., Carlsson, G., Tallkvist, J., Norrgren, L., Effect of perfluorooctanoic acid (PFOA) on zebrafish (Danio rerio) reproduction and sexual development. (Manuscript)
Place of Publication:Uppsala
Publisher:Dept. of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences
Associated Programs and Other Stakeholders:SLU - Research Areas for the Future > Future Animal Health and Welfare (until Jan 2017)
ISBN for printed version:978-91-576-7928-4
ISBN for electronic version:978-91-576-7929-1
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L53 Animal physiology - Reproduction
T Pollution > T01 Pollution
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology
Agrovoc terms:danio rerio, organic compounds, toxic substances, toxicity, reproduction, reproductive performance, animal embryos, biological development, locomotion
Keywords:zebrafish, perfluoroalkyl acids, toxicity, reproduction, early development, kinetic
Permanent URL:
ID Code:10964
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: Mr Mazhar Ul Haq
Deposited On:08 Jan 2014 08:43
Metadata Last Modified:02 Dec 2014 11:04

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