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Verticillium longisporum and plant immunity responses in Arabidopsis

Roos, Jonas (2014). Verticillium longisporum and plant immunity responses in Arabidopsis. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2014:14
ISBN 978-91-576-7976-5
eISBN 978-91-576-7977-2
[Doctoral thesis]

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Abstract

Verticillium spp. are soil-borne ascomycete fungi belonging to a subgroup of Sordariomycetes, and the three major plant pathogens Verticillium longisporum, V. dahliae and V. albo-atrum cause disease on numerous plant species worldwide. In Sweden, V. longisporum poses a threat to Brassica oilseed crops, and is thus emphasized in this thesis. Here the early immune responses to V. longisporum in the model plant Arabidopsis and recent data on the V. longisporum genome are presented.

Three genes of importance in the Arabidopsis–V. longisporum interaction were studied. The genes were identified via transcriptome and single nucleotide polymorphism (SNP) analysis. RabGAP22, a RabGTPase-regulating protein, was found to contribute to V. longisporum resistance. Pull-down assays revealed SERINE:GLYOXYLATE AMINOTRANSFERASE (AGT1) as an interacting partner during V. longisporum infection and the two proteins were shown to co-localize in the peroxisomes. Unexpectedly, a role for RabGAP22 was also found in stomatal immunity. The monoterpene synthase TPS23/27 was on the other hand found to contribute to fungal invasion, by triggering germination of V. longisporum conidia. The third gene codes for a nitrate/peptide transporter, NPF5.12. Pull-down experiments and fluorescent imaging revealed interaction between NPF5.12 and a major latex protein family member, NPFBP1. Implications in plant immunity processes of these three genes are further discussed.

The genomes of two Swedish V. longisporum isolates were sequenced and found to have a size of approximately 70 Mb and harbor ~21,000 protein-coding genes. Initial analyses revealed that 86% of the V. longisporum genomes are shared with V. dahliae and V. albo-atrum, with a high extent of gene duplications. Large numbers of proteins were predicted to contain secretion motifs, and this group of proteins is presumed to play major roles in the interactions with V. longisporum host plants.

In conclusion, this thesis work has revealed new fungal and plant host genes and thereby laid the basis for new plant breeding and disease protection strategies.

Authors/Creators:Roos, Jonas
Title:Verticillium longisporum and plant immunity responses in Arabidopsis
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :14 February 2014
Volume:2014:14
Number of Pages:55
Papers/manuscripts:
NumberReferences
I.Roos, J., Bejai, S., Oide, S. and Dixelius, C. (2014) RabGAP22 is required for defense to the vascular pathogen Verticillium longisporum and contributes to stomata immunity. PLoS ONE, 9, e88187.
II.Roos, J., Bejai, S., Dixelius, C. (2014) Arabidopsis thaliana monoterpene synthase TPS23/27 triggers infection by the fungal pathogen Verticillium longisporum (manuscript).
III.Roos, J., Bejai, S. and Dixelius, C. (2014) AtNPF5.12 is a nitrate/peptide transporter involved in the defense response to the fungal pathogen Verticillium longisporum. (manuscript).
IV.Fogelqvist, J., Bejai, S., Kamber, T., Roos, J., Schwelm, A. and Dixelius, C. (2014) The first glimpse of the Verticillium longisporum genome. (manuscript).
Place of Publication:Uppsala
Publisher:Dept. of Plant Biology, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-7976-5
ISBN for electronic version:978-91-576-7977-2
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
H Protection of plants and stored products > H20 Plant diseases
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
Agrovoc terms:arabidopsis thaliana, pathogens, brassicaceae, immunity, verticillium longisporum, fungal diseases, host pathogen relations, defence mechanisms, terpenoids, genomes, genes, nucleotide sequence
Keywords:Arabidopsis thaliana, immunity, pathogen, Rab, terpene, Verticillium longisporum
URN:NBN:urn:nbn:se:slu:epsilon-e-1784
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1784
ID Code:11007
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Plant Biology (from 140101)
External funders:FORMAS
Deposited By: Jonas Roos
Deposited On:17 Feb 2014 08:58
Metadata Last Modified:19 Feb 2015 14:17

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