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Single layer centrifugation-selected boar spermatozoa are capable of fertilization in vitro

Brandt, Ylva and Morrell, Jane and Gonzalez Herrero, Raquel (2013). Single layer centrifugation-selected boar spermatozoa are capable of fertilization in vitro. Acta veterinaria Scandinavica. 55:20, 1-6
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Official URL: http://dx.doi.org/10.1186/1751-0147-55-20

Abstract

BACKGROUND: Good quality spermatozoa are important to achieve fertilization, viable embryos and offspring. Single
Layer Centrifugation (SLC) through a colloid (Androcoll-P) selects good quality spermatozoa. However, it has not
been established previously whether porcine spermatozoa selected by this method maintain their fertility.

METHODS: The semen was prepared either by SLC or by standard centrifugation (control) and used for in vitro
fertilization (IVF) at oocyte:spermatozoa ratios of 1:50; 1:100 and 1:300 (or 4 x 103, 8 x 103 and 24 x 103
spermatozoa/ml) to evaluate their subsequent ability to generate blastocysts. In addition, sperm motility was
assessed by computer assisted sperm motility analysis.

RESULTS: Total and progressive motility were significantly higher in sperm samples prepared by SLC compared to
uncentrifuged samples. Sperm binding ability, polyspermy, cleavage and blastocyst rates were affected by the
oocyte:sperm ratio, but not by sperm treatment.

CONCLUSION: The use of SLC does not adversely affect the in vitro fertilizing and embryo-generating ability of the
selected spermatozoa compared to their unselected counterparts, but further modifications in the IVF conditions
would be needed to improve the monospermy in IVF systems. Since SLC did not appear to have a negative effect
on sperm fertilizing ability, and may in fact select for spermatozoa with a greater potential for fertilization, an in vivo trial to determine the usefulness of this sperm preparation technique prior to artificial insemination is warranted.

Authors/Creators:Brandt, Ylva and Morrell, Jane and Gonzalez Herrero, Raquel
Title:Single layer centrifugation-selected boar spermatozoa are capable of fertilization in vitro
Series/Journal:Acta veterinaria Scandinavica (0044-605X)
Year of publishing :2013
Volume:55
Number:20
Page range:1-6
Number of Pages:8
Publisher:BioMed Central
ISSN:0044-605X
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:L Animal production > L53 Animal physiology - Reproduction
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Agrovoc terms:Pigs, Semen, Sperm, Selection
Keywords:IVF, Pig, Semen, Sperm selection
ID Code:11025
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:24 Mar 2014 14:39
Metadata Last Modified:20 Apr 2015 11:50

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