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PGP expression in Cooperia oncophora before and after ivermectin selection

Areskog, Marlene and Engström, Annie and Tallkvist, Jonas and von Samson-Himmelstjerna, Georg and Höglund, Johan (2013). PGP expression in Cooperia oncophora before and after ivermectin selection. Parasitology research. 112:8, 3005-3012
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Abstract

The aim of this study was to investigate genetic selection and P-glycoprotein (PGP) expression in three different isolates of Cooperia oncophora before treatment and after ivermectin (IVM) injection. Adult parasites were recovered from nine calves experimentally infected with the isolates represented by one IVM susceptible laboratory isolate, and two field isolates showing signs of phenotypic macrocyclic lactone resilience according to the faecal egg count reduction test. Five males and five females per isolate were examined both pre- and post-IVM treatment giving a total of 60 worms. A sequence from C. oncophora (Con-pgp) was identified, showing 83% similarity to Pgp-9 of Caenorhabditis elegans. Primers specific to putative Con-pgp-9 mRNA were designed, generating a 153-bp PCR product. Total RNA was prepared from all worms, and Con-pgp-9 expression was measured by quantitative real-time reverse transcription PCR. Our results showed that mean PGP concentrations were four to five times higher in female as compared to male worms. No significant differences in gene expression between experimental groups pre- and post-IVM selection were detected. However, PGP gene expression tended to be increased by IVM treatment in male worms (p = 0.091), with 70% higher mean expression in treated than in untreated male worms. Amplified fragment length polymorphism analysis did not demonstrate any bottleneck effect within the different isolates post-treatment. The total mean gene diversity values were 0.158 and 0.153 before and after treatment, respectively. Inbreeding coefficient in subpopulations compared to total population F ST was 0.0112, suggesting no genetic differentiation between or within the investigated isolates in relation to treatment. In conclusion, comparison of Con-pgp-9 expression showed no significant difference before and after treatment, but some tendency towards increasing expression in male worms.

Authors/Creators:Areskog, Marlene and Engström, Annie and Tallkvist, Jonas and von Samson-Himmelstjerna, Georg and Höglund, Johan
Title:PGP expression in Cooperia oncophora before and after ivermectin selection
Series/Journal:Parasitology research (1432-1955)
Year of publishing :2013
Volume:112
Number:8
Page range:3005-3012
Number of Pages:8
Publisher:Springer Berlin Heidelberg
ISSN:1432-1955
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology
Keywords:microbiology, immunology
URN:NBN:urn:nbn:se:slu:epsilon-e-1893
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1893
Additional ID:
Type of IDID
Web of Science (WoS)000322441000026
DOI10.1007/s00436-013-3473-5
ID Code:11086
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: SLUpub Connector
Deposited On:13 May 2014 06:54
Metadata Last Modified:08 Mar 2016 15:23

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