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Cadmium transport in a model of neonatal intestinal cells correlates to MRP1 and not DMT1 or FPN1

Öhrvik, Helena and Tyden, Eva and Artursson, Per and Tallkvist, Jonas and Oskarsson, Agneta (2013). Cadmium transport in a model of neonatal intestinal cells correlates to MRP1 and not DMT1 or FPN1. . 2013, 1-9
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Official URL: http://dx.doi.org/10.1155/2013/892364

Abstract

Newborns have a higher gastrointestinal uptake of cadmium than adults. In adults, the iron transporters DMT1 and FPN1 are involved in the intestinal absorption of cadmium, while in neonates, the mechanisms for cadmium absorption are unknown. We have investigated possible cadmium transporters in the neonatal intestine by applying a model of immature human intestinal epithelial Caco-2 cells. To mimic the continuous cadmium exposure via diet in neonates, cells were allowed to differentiate for 7 days in medium containing 1 μM CdCl2. A dramatic upregulation of the MT1 gene expression followed cadmium pretreatment, indicating a high sensitivity of the immature cells to cadmium. Cadmium pretreatment increased the basolateral efflux of109Cd, without causing any effects on the passive diffusion of mannitol or the transepithelial electrical resistance. The augmented transport of cadmium was correlated to an upregulation of MRP1 gene expression and increased activity of the efflux protein MRP1. No effects were observed on gene expression of the efflux proteins MRP2 and P-gp or the iron transporters DMT1, DMT1-IRE and FPN1. In conclusion, our data indicate that continuous cadmium exposure increases the absorption of the metal in immature intestinal cells and that MRP1 is involved in the intestinal cadmium absorption in newborns.

Authors/Creators:Öhrvik, Helena and Tyden, Eva and Artursson, Per and Tallkvist, Jonas and Oskarsson, Agneta
Title:Cadmium transport in a model of neonatal intestinal cells correlates to MRP1 and not DMT1 or FPN1
Series/Journal:2090-6188, ISRN Toxicology
Year of publishing :2013
Volume:2013
Page range:1-9
Number of Pages:9
Publisher:Hindawi Publishing Corporation
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:X Agricola extesions > X38 Human medicine, health, and safety
X Agricola extesions > X50 Chemistry
Subjects:(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 301 Basic Medicine > Pharmacology and Toxicology
Agrovoc terms:Cadmium, Intestinal absorption, Cells, Toxicity, Gastrointestinal system
Keywords:Cadmium transport, Intestinal cells, Newborns, Cadmium toxicity, Gastrointestinal absorption, Cadmium exposure
URN:NBN:urn:nbn:se:slu:epsilon-e-1913
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1913
Additional ID:
Type of IDID
DOI10.1155/2013/892364
ID Code:11092
Faculty:V - Veterinärmedicinsk fakultet (t.o.m. 2003)
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: SLUpub Connector
Deposited On:20 May 2014 13:08
Metadata Last Modified:11 Apr 2015 19:10

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