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Characterisation of chlorinated fatty acid metabolites in human cells after uptake of dichlorostearic acid, as determined by a halogenspecific detector (XSD)

Åkesson Nilsson, Gunilla (2000). Characterisation of chlorinated fatty acid metabolites in human cells after uptake of dichlorostearic acid, as determined by a halogenspecific detector (XSD). Uppsala : Sveriges lantbruksuniv. , Rapport / Sveriges lantbruksuniversitet, Miljöanalys, 1403-977X ; 2000:9
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[Licentiate thesis]

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Abstract

Chlorinated fatty acids (CIFAs) have been found to account for a considerable portion of the
extractable organically bo und chlorine in aquatic animals. Methods particularly suitable for the
determination of CIFAs have relatively newly been developed, which may explain why these
compounds did not come to attention as a potentially hazardous environmental pollutant until during
the recent decade. The presence of CIFAs in human tissues has not yet been reported, and it is not
known if human cells can incorporate and metabolise these compounds.

Recently a new halogen specific detector (XSD) has been introduced. It is described to be a
sensitive, selective, and robust detector in the determination of halogenated pesticides. It has been
declared to be more stable and easier to maintain than other halogen selective detectors. The XSD
operates in an oxidative pyrolysis mode and the sample compounds are converted into their
oxidation products. When halogen-containing compounds enter the hot detector, the detector current
will increase. CIF As have not earlier been studied by an XSD.

The aim of this work was to study i) the XSD, connected to a gas chromatograph, in the
determination of CIFA methyl esters (CIFAMEs), and ii) the incorporation and metabolism of
CIFAs in human cell lines, as determined by XSD detection.

It was shown that the XSD provides a detection limit (0.2 ng of dichlorostearic acid methyl
ester) and a selectivity ((response CIFAMEs)/(response unchlorinated fally acid methyl esters)) >>
10⁴ similar to that of the electrolytic conductivity detector (ELCD), commonly used in
determination of CIFAMEs. Furthermore, the XSD was found to be very easy and stable to maintain
in the analysis of CIFAMEs.

In the determination of CIFAs obtained from human cell lines, it was established that human
cells can incorporate dichlorostearic acid and degrade it to dichloropalmitic acid and
dichloromyristic acid, probably through α3-oxidation. CIFAs were found both in the neutrallipid and
in the phospholipid fraction of the cultured cells. Dichloromyristic acid was the shortest CIFAs
detected and was found to be released to the culture medium to a higher extent than the other
CIFAs.

The XSD was found to be a good alternative to the ELCD in the determination of CIFAs and the
XSD is suitable for use in continued studies of the turnover of CIFAs in human cells.

Authors/Creators:Åkesson Nilsson, Gunilla
Title:Characterisation of chlorinated fatty acid metabolites in human cells after uptake of dichlorostearic acid, as determined by a halogenspecific detector (XSD)
Series/Journal:Rapport / Sveriges lantbruksuniversitet, Miljöanalys
Year of publishing :2000
Volume:2000:9
Number of Pages:23
Papers/manuscripts:
NumberReferences
I.Åkesson-Nilsson, G., Nilsson, O., Odenbrand, 1., and Wesen, C. A new halogen-specific detector, XSD, used for the analysis of chlorinated fatt y acids. J. Chromatogr. A, under revision.
II.Gustafson-Svärd, C., Åkesson-Nilsson, G., Mattsson, M., Sundin, P., and Wesen, C. Removal of xenobiotic dichlorostearic acid from phospholipids and neutrallipids in cultured human cell lines by f3-oxidation and secretion of dichloromyristic acid. Submitted to Pharmacology & Toxicology.
Place of Publication:Uppsala
Publisher:Institutionen för miljöanalys, Sveriges lantbruksuniversitet
ISBN for printed version:-
ISSN:1403-977X
Language:English
Publication Type:Licentiate thesis
Full Text Status:Public
Agris subject categories.:T Pollution > T01 Pollution
X Agricola extesions > X50 Chemistry
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 104 Chemical Sciences > Organic Chemistry
Agrovoc terms:organic halogen compounds, halogens, fatty acids, metabolites, phospholipids
Keywords:halogenated fatty acids, halogen-selective detection, phospholipids, neutral lipids, dichloropalmitic acid, dichloromyristic acid, beta-oxidation
URN:NBN:urn:nbn:se:slu:epsilon-e-1894
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1894
ID Code:11174
Faculty:L - Lantbruksvetenskaplig fakultet (t.o.m. 2003)
Department:(NL, NJ) > Dept. of Environmental Assessment
Deposited By: Gunilla Åkerlund
Deposited On:15 May 2014 06:08
Metadata Last Modified:24 Mar 2015 07:41

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