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Degradation of polycyclic aromatic hydrocarbons by actinomycetes

Pizzul, Leticia (2006). Degradation of polycyclic aromatic hydrocarbons by actinomycetes. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2006:50
ISBN 91-576-7099-4
[Doctoral thesis]

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The potential of some actinomycetes to degrade polycyclic aromatic hydrocarbons (PAH) and the effect of co-substrates, plants and other additives on their degradation and bioavailability was studied. A glass bead system for growth of PAH-degrading actinomycetes in liquid culture was developed and used for the screening of strains for biosurfactant activity and phenanthrene degradation in the presence of different co-substrates. Indication of biosurfactant production by all tested strains was obtained with hexadecane and rapeseed oil as co-substrates but not with glucose. Rhodococcus sp. DSM 44126 was identified as R. wratislaviensis and found to be able to degrade phenanthrene and anthracene. An actinomycete with a high capacity to degrade phenanthrene and pyrene was isolated from an agricultural soil and identified as Mycobacterium LP1. The catabolic activity of both strains was studied in liquid cultures and in soil. Several additives were also tested for their effect on PAH degradation in soils. The surfactant Triton X-100, but not wheat straw, promoted PAH degradation in a soil with aged creosote by increasing the bioavailability of the compounds. The presence of plants increased the proportion of active microorganisms and enhanced PAH degradation, likely due to root exudates provided by the plants. In a PAH-spiked soil, the addition of rapeseed oil (1% w/w) stimulated the degradation of anthracene and benzo(a)pyrene mainly as a result of abiotic processes, but negatively affected the degradation of phenanthrene and pyrene, probably due to limitations in nutrient and oxygen supply. Based on these results, a new sequential treatment in two steps for cleaning PAH-contaminated soil was designed and tested using four different PAH as model substances. The first step consisted of the inoculation with Mycobacterium LP1, favouring biological degradation of low-molecular-weight PAH, and the second step consisted of the addition of rapeseed oil, which promoted the abiotic transformation, and probably also the solubilisation, of the high-molecular-weight PAH.

Authors/Creators:Pizzul, Leticia
Title:Degradation of polycyclic aromatic hydrocarbons by actinomycetes
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :May 2006
Number of Pages:39
ALLI. Pizzul, L., Castillo, M.d.P. & Stenström, J. 2006. Characterization of selected actinomycetes degrading polyaromatic hydrocarbons in liquid culture and spiked soil. World Journal of Microbiology & Biotechnology, in press DOI 10.1007/s11274-005-9100-6. II. Pizzul, L., Castillo, M.d.P. & Stenström, J. Effect of rapeseed oil on the degradation of PAH in soil by Rhodococcus wratislaviensis. (In revision with International Biodeterioration & Biodegradation). III. Pizzul, L., Sjögren, Å, Castillo, M.d.P. & Stenström, J. Degradation of polyaromatic hydrocarbons in soil by a two-step sequential treatment. (Manuscript). IV. Hultgren, J., Granhall, U., Pizzul, L & Castillo, M.d.P. Phytoremediation of polycyclic aromatic hydrocarbons by means of Salix viminalis - a greenhouse experiment with creosote contaminated soils. (Manuscript).
Place of Publication:Uppsala
ISBN for printed version:91-576-7099-4
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:bioremediation, biodegradation, aromatic hydrocarbons, polluted soil, mycobacterium, rhodococcus (bacteria), bioavailability, rapeseed oil
Keywords:bioavailability, bioremediation, co-substrate, Mycobacterium, PAH, phytoremediation, polycyclic aromatic hydrocarbons, rapeseed oil, Rhodococcus
Permanent URL:
ID Code:1118
Department:(NL, NJ) > Dept. of Microbiology (until 161231)
Deposited By: Leticia Pizzul
Deposited On:12 May 2006 00:00
Metadata Last Modified:02 Dec 2014 10:09

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