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Metagenomic Detection of Viral Pathogens in Spanish Honeybees: Co- Infection by Aphid Lethal Paralysis, Israel Acute Paralysis and Lake Sinai Viruses

Granberg, Fredrik and Vicente-Rubiano, Marina and Rubio-Guerri, Consuelo and Karlsson, Oskar and Kukielka, Deborah and Belak, Sandor and Sánchez-Vizcaíno, José Manuel (2013). Metagenomic Detection of Viral Pathogens in Spanish Honeybees: Co- Infection by Aphid Lethal Paralysis, Israel Acute Paralysis and Lake Sinai Viruses. PloS one. 8:2, 1-8
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Official URL: http://dx.doi.org/10.1371/journal.pone.0057459

Abstract

The situation in Europe concerning honeybees has in recent years become increasingly aggravated with steady decline in populations and/or catastrophic winter losses. This has largely been attributed to the occurrence of a variety of known and "unknown", emerging novel diseases. Previous studies have demonstrated that colonies often can harbour more than one pathogen, making identification of etiological agents with classical methods difficult. By employing an unbiased metagenomic approach, which allows the detection of both unexpected and previously unknown infectious agents, the detection of three viruses, Aphid Lethal Paralysis Virus (ALPV), Israel Acute Paralysis Virus (IAPV), and Lake Sinai Virus (LSV), in honeybees from Spain is reported in this article. The existence of a subgroup of ALPV with the ability to infect bees was only recently reported and this is the first identification of such a strain in Europe. Similarly, LSV appear to be a still unclassified group of viruses with unclear impact on colony health and these viruses have not previously been identified outside of the United States. Furthermore, our study also reveals that these bees carried a plant virus, Turnip Ringspot Virus (TuRSV), potentially serving as important vector organisms. Taken together, these results demonstrate the new possibilities opened up by high-throughput sequencing and metagenomic analysis to study emerging new diseases in domestic and wild animal populations, including honeybees.

Authors/Creators:Granberg, Fredrik and Vicente-Rubiano, Marina and Rubio-Guerri, Consuelo and Karlsson, Oskar and Kukielka, Deborah and Belak, Sandor and Sánchez-Vizcaíno, José Manuel
Title:Metagenomic Detection of Viral Pathogens in Spanish Honeybees: Co- Infection by Aphid Lethal Paralysis, Israel Acute Paralysis and Lake Sinai Viruses
Series/Journal:PloS one (1932-6203)
Year of publishing :2013
Volume:8
Number:2
Page range:1-8
Number of Pages:8
Publisher:Public Library of Science
ISSN:1932-6203
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology
Keywords:honeybees, pathogens, viruses, diseases, metagenomic analysis, Spain
URN:NBN:urn:nbn:se:slu:epsilon-e-1996
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1996
Additional ID:
Type of IDID
Web of Science (WoS)000315519000114
DOI10.1371/journal.pone.0057459
ID Code:11250
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: SLUpub Connector
Deposited On:03 Jul 2014 08:17
Metadata Last Modified:24 Jan 2015 01:51

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