Anees, Muhammad and Zubair Shabbir, Muhammad and Muhammad, Khushi and Nazir, Jawad and Abu Bakar Shabbir, Muhammad and Wensman, Jonas Johansson and Munir, Muhammad
(2013).
Genetic analysis of peste des petits ruminants virus from Pakistan.
BMC Veterinary Research. 9
, 1-5
[Research article]
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Abstract
BACKGROUND: Peste des petits ruminants (PPR) is an endemic and highly contagious disease in small ruminants of Pakistan. Despite the fact that an effective vaccine is available, outbreaks are regularly occurring in the country. Thus so far, the diagnosis has primarily been made based on clinical outcome or serology. This study was carried out to characterize PPRV from an emerging wave of outbreaks from Punjab, Pakistan. RESULTS: A total of 32 blood samples from five different flocks were tested with real-time PCR for the presence of PPRV genome. The samples detected positive in real-time PCR (n = 17) were subjected to conventional PCR for the amplification of the nucleoprotein (N) gene. Phylogenetic analysis of the sequenced N genes (n = 8) indicated the grouping of all the sequences in lineage IV along with PPRV strains from Asian and Middle East. However, interestingly sequences were divided into two groups. One group of viruses (n = 7) clustered with previously characterized Pakistani isolates whereas one strain of PPRV was distinct and clustered with Saudi Arabian and Iranian strains of PPRV. CONCLUSIONS: Results demonstrated in this study expanded the information on the genetic nature of different PPRV population circulating in small ruminants. Such information is essential to understand genetic nature of PPRV strains throughout the country. Proper understanding of these viruses will help to devise control strategies in PPRV endemic countries such as Pakistan.
Authors/Creators: | Anees, Muhammad and Zubair Shabbir, Muhammad and Muhammad, Khushi and Nazir, Jawad and Abu Bakar Shabbir, Muhammad and Wensman, Jonas Johansson and Munir, Muhammad | ||||||
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Title: | Genetic analysis of peste des petits ruminants virus from Pakistan | ||||||
Series Name/Journal: | BMC Veterinary Research | ||||||
Year of publishing : | 2013 | ||||||
Volume: | 9 | ||||||
Page range: | 1-5 | ||||||
Number of Pages: | 5 | ||||||
Publisher: | BioMed Central Ltd. | ||||||
ISSN: | 1746-6148 | ||||||
Language: | English | ||||||
Publication Type: | Research article | ||||||
Refereed: | Yes | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution-Noncommercial 3.0 | ||||||
Full Text Status: | Public | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology | ||||||
Keywords: | Morbillivirus, Peste des petits ruminants, PPR, diseases, Pakistan | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-e-1997 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1997 | ||||||
Additional ID: |
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Alternative URL: | http://www.biomedcentral.com/1746-6148/9/60 | ||||||
ID Code: | 11257 | ||||||
Faculty: | VH - Faculty of Veterinary Medicine and Animal Science | ||||||
Department: | (VH) > Dept. of Clinical Sciences (VH) > Dept. of Biomedical Sciences and Veterinary Public Health | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 03 Jul 2014 08:44 | ||||||
Metadata Last Modified: | 12 Jul 2021 10:28 |
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