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Regulation of genes related to lipid metabolism in Atlantic salmon (Salmo salar L.)

in vitro and in vivo studies

Schiller Vestergren, AnnaLotta (2012). Regulation of genes related to lipid metabolism in Atlantic salmon (Salmo salar L.). Uppsala : Sveriges lantbruksuniv. , Rapport / Sveriges lantbruksuniversitet, Institutionen för livsmedelsvetenskap, 1101-5411 ; 28
ISBN 978-91-576-9061-6
eISBN 978-91-576-9112-5
[Licentiate thesis]

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Abstract

Fish is a vital source of highly valuable omega-3 (n-3) fatty acids (FA) in the human diet. With declining commercial fisheries, aquaculture fish constitute a growing proportion in human consumption. Sustainable development of aquaculture dictates that the fish feed used not solely is based on fish oil (FO) but also contain increasing levels of vegetable oil (VO). The replacement of FO with VO influences FA composition in fish tissues with the decrease of the n-3 highly unsaturated fatty acids (HUFAs), as main consequence rendering a fish less beneficial for human health. Accordingly the last decade of salmonid research has been focusing on increasing the amount of HUFAs in fish fed VO diets. Part of this focus has been on the addition of bioactive compounds to VO diets.

In this thesis 2 studies are presented trying to shed more light on the potential positive effects of bioactive compounds.

Paper I examine in vivo the effects of sesamin inclusion on Atlantic salmon (Salmo salar L.) fed VO-based diets with different n-6/n-3 FA ratios. Fish were fed for 4 months.

In Paper II in vitro effects of bioactive compounds (genistein, lipoic acid, sesamin/episesamin and sesamin) were investigated in Atlantic salmon primary hepatocytes. Analyses were made 12h and 48h after addition of bioactive compounds, with most effects seen after 48h. In both studies, the FA composition and expression of genes involved in transcription, lipid uptake, desaturation, elongation and β-oxidation were examined.

Genes involved in transcription regulation, β-oxidation, elongation and desaturation were affected by addition of bioactive compounds in both in vivo and in vitro experiments. Effects on the FA composition were found, but no clear affect on the DHA content. High level of sesamin supplementation had negative effect on growth rate and live weight.

Authors/Creators:Schiller Vestergren, AnnaLotta
Title:Regulation of genes related to lipid metabolism in Atlantic salmon (Salmo salar L.)
Subtitle:in vitro and in vivo studies
Series/Journal:Rapport / Sveriges lantbruksuniversitet, Institutionen för livsmedelsvetenskap (1101-5411)
Year of publishing :14 December 2012
Volume:28
Number of Pages:77
Papers/manuscripts:
NumberReferences
I.Schiller Vestergren, A., Wagner, L., Pickova, J., Rosenlund, G., Kamal-Eldin, A., Trattner, S. (2012). Sesamin modulates gene expression without corresponding effect on fatty acids in Atlantic salmon (Salmo salar L.). Lipids 47(9), 897-911.
II.Schiller Vestergren, A., Trattner, S., Mráz, J. Ruyter, B., Pickova, J. (2011). Fatty acids and gene expression responses to bioactive compounds in Atlantic salmon (Salmo salar L.) hepatocytes. Neuroendocrinology Letters 32(Suppl. 2), 41-50.
Place of Publication:Uppsala
Publisher:Dept. of Food Science, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-9061-6
ISBN for electronic version:978-91-576-9112-5
ISSN:1101-5411
Language:English
Additional Information:ISBN for printed version is invalid. 978-91-576-9061-6 is associated with another publication.
Publication Type:Licentiate thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L50 Animal physiology and biochemistry
Q Food science > Q04 Food composition
S Human nutrition > S01 Human nutrition - General aspects
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Food Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Fish and Aquacultural Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
Agrovoc terms:salmo salar, atlantic salmon, fish feeding, lipids, lipid metabolism, genes, gene expression, fatty acids, adipose tissue, growth
Keywords:β-oxidation, elongation, desaturation, gene expression, sesamin, episesamin, lipoic acid, genistein, DHA, n-6/n-3 fatty acid ratio
URN:NBN:urn:nbn:se:slu:epsilon-e-2116
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2116
ID Code:11457
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Food Science (until 161231)
Deposited By: Ms AnnaLotta Schiller Vestergren
Deposited On:11 Sep 2014 10:31
Metadata Last Modified:17 Feb 2015 00:23

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