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Identification of host volatiles and their role in the behavioural modulation of host-seeking Culicoides biting midges

Isberg, Elin (2014). Identification of host volatiles and their role in the behavioural modulation of host-seeking Culicoides biting midges. Diss. (sammanfattning/summary) Alnarp : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2014:86
ISBN 978-91-576-8120-1
eISBN 978-91-576-8121-8
[Doctoral thesis]

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Abstract

Culicoides biting midges are important vectors of Bluetongue and Schmallenberg viruses in Europe. The introduction of these viruses highlighted the need to develop novel surveillance and control tools to monitor and manage biting midges.

Biting midges, as most insects, primarily use olfactory cues to recognize and discriminate resources for their survival and reproduction. Blood feeding insects rely on host-derived volatiles to locate their vertebrate hosts, and these odours can be exploited to manipulate insect-host interaction. The primary goal of this thesis was to identify host volatiles that modulate the host-seeking behaviour of biting midges. Through coupled gas chromatography and electroantennographic detection (GC-EAD) analysis of odour collections from cattle hair and urine, I identified several compounds that, in combination with carbon dioxide, elicited behavioural attraction or inhibition in Culicoides nubeculosus, in a dose dependent manner. Some of these host odours were evaluated in the field showing similar behavioural responses of C. impunctatus. Both laboratory and field assays emphasized the importance of release rate to obtain optimal behavioural responses.

The impact of using host volatiles to monitor host-seeking biting midges was shown in another study. Here, I showed that incandescent light traps baited with two cattle kairomones, 1-octen-3-ol and carbon dioxide, caught significantly more insects than UV light traps. UV light traps are to date the most commonly used method for monitoring biting midges. That said, differences were observed between trap types, both concerning species composition and the physiological state of the female biting midges caught. Incorporating the novel bioactive compounds, formulated in blends and released in proper ratio and rate, could further increase the efficacy of this trapping method. By incorporating these attractants together with repellent compounds identified in this project, a "push-pull"- system for biting midges could also be envisioned.

The host volatiles identified are detected by the peripheral olfactory system of biting midges. A comparative analysis across biting species with different host preferences revealed a correlation between host preference and the number and types of antennal and maxillary palp sensilla. Functional characterisation of these sensillum types will be the next step to increase our understanding of how host odours are detected by biting midges

With this thesis I have increased our understanding of the chemical ecology of host-seeking female biting midges and their peripheral olfactory system. Future research projects will need to develop viable commercial products that could be incorporated into management strategies for Culicoides biting midges.

Authors/Creators:Isberg, Elin
Title:Identification of host volatiles and their role in the behavioural modulation of host-seeking Culicoides biting midges
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :16 October 2014
Volume:2014:86
Number of Pages:60
Papers/manuscripts:
NumberReferences
I.Elin Isberg, Ylva Hillbur, Rickard Ignell (2013). Numbers of antennal and maxillary palp sensilla in the context of phylogeny and host preference of biting midges (Culicoides: Ceratopogonidae). Journal of Medical Entomology 50(3), 486-492.
II.Elin Isberg, Ylva Hillbur, Rickard Ignell. Evaluation of light- and odor-baited traps for capturing Culicoides biting midges. Manuscript.
III.Elin Isberg, Ylva Hillbur, Göran Birgersson, Rickard Ignell. Identification of cattle-derived volatiles that modulate the behavioural response of the biting midge Culicoides nubeculosus (Diptera: Ceratopognidae). Manuscript
IV.Elin Isberg, Ylva Hillbur, Rickard Ignell. Evaluation of host-derived volatiles for trapping Culicoides biting midges. Manuscript
Place of Publication:Alnarp
Publisher:Dept. of Plant Protection Biology, Swedish University of Agricultural Sciences
Associated Programs and Other Stakeholders:SLU - Agricultural Sciences for Global Development > Efficiency in farming systems
ISBN for printed version:978-91-576-8120-1
ISBN for electronic version:978-91-576-8121-8
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L20 Animal ecology
X Agricola extesions > X38 Human medicine, health, and safety
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Ecology
(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 303 Health Sciences > Public Health, Global Health, Social Medicine and Epidemiology
Agrovoc terms:culicoides, midges, ceratopogonidae, olfactory organs, hosts, smell, viruses, vectors, disease transmission, gas chromatography
Keywords:Biting midges, Olfaction, Host odours, GC-EAD, Sensilla
URN:NBN:urn:nbn:se:slu:epsilon-e-2167
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2167
ID Code:11595
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Protection Biology
External funders:FORMAS
Deposited By: Elin Isberg
Deposited On:16 Oct 2014 11:34
Metadata Last Modified:02 Dec 2014 11:09

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