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Starch biosynthetic genes and enzymes are expressed and active in the absence of starch accumulation in sugar beet tap-root

Turesson, Helle and Andersson, Mariette and Marttila, Salla and Thulin, Ingela and Hofvander, Per (2014). Starch biosynthetic genes and enzymes are expressed and active in the absence of starch accumulation in sugar beet tap-root. BMC plant biology. 14:104, 1-12
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Abstract

BACKGROUND: Starch is the predominant storage compound in underground plant tissues like roots and tubers. An exception is sugar beet tap-root (Beta vulgaris ssp altissima) which exclusively stores sucrose. The underlying mechanism behind this divergent storage accumulation in sugar beet is currently not fully known. From the general presence of starch in roots and tubers it could be speculated that the lack in sugar beet tap-roots would originate from deficiency in pathways leading to starch. Therefore with emphasis on starch accumulation, we studied tap-roots of sugar beet using parsnip (Pastinaca sativa) as a comparator. RESULTS: Metabolic and structural analyses of sugar beet tap-root confirmed sucrose as the exclusive storage component. No starch granules could be detected in tap-roots of sugar beet or the wild ancestor sea beet (Beta vulgaris ssp. maritima). Analyses of parsnip showed that the main storage component was starch but tap-root tissue was also found to contain significant levels of sugars. Surprisingly, activities of four main starch biosynthetic enzymes, phosphoglucomutase, ADP-glucose pyrophosphorylase, starch synthase and starch branching enzyme, were similar in sugar beet and parsnip tap-roots. Transcriptional analysis confirmed expression of corresponding genes. Additionally, expression of genes involved in starch accumulation such as for plastidial hexose transportation and starch tuning functions could be determined in tap-roots of both plant species. Conclusion: Considering underground storage organs, sugar beet tap-root upholds a unique property in exclusively storing sucrose. Lack of starch also in the ancestor sea beet indicates an evolved trait of biological importance. Our findings in this study show that gene expression and enzymatic activity of main starch biosynthetic functions are present in sugar beet tap-root during storage accumulation. In view of this, the complete lack of starch in sugar beet tap-roots is enigmatic.

Authors/Creators:Turesson, Helle and Andersson, Mariette and Marttila, Salla and Thulin, Ingela and Hofvander, Per
Title:Starch biosynthetic genes and enzymes are expressed and active in the absence of starch accumulation in sugar beet tap-root
Series/Journal:BMC plant biology (10381464)
Year of publishing :2014
Volume:14
Number:104
Page range:1-12
Number of Pages:12
Publisher:BioMed Central
Language:English
Additional Information:Bio Med Central license agreement: http://www.biomedcentral.com/about/license
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
X Agricola extesions > X30 Life sciences
X Agricola extesions > X50 Chemistry
X Agricola extesions > X60 Technology
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
Agrovoc terms:Beta vulgaris, Pastinaca sativa, carbon, starch, sucrose
Keywords:Beta vulgaris, Pastinaca sativa, storage accumulation, carbon allocation, starch, sucrose
URN:NBN:urn:nbn:se:slu:epsilon-e-2245
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2245
Additional ID:
Type of IDID
Web of Science (WoS)000335992000001
DOI10.1186/1471-2229-14-104
ID Code:11604
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Chemistry and Biotechnology (140101-161231)
Deposited By: SLUpub Connector
Deposited On:27 Nov 2014 17:07
Metadata Last Modified:14 Dec 2014 13:04

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