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Carbon allocation in underground storage organs

studies on accumulation of starch, sugars and oil

Turesson, Helle (2014). Carbon allocation in underground storage organs. Diss. (sammanfattning/summary) Alnarp : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2014:100
ISBN 978-91-576-8148-5
eISBN 978-91-576-8149-2
[Doctoral thesis]

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Abstract

By increasing knowledge of carbon allocation in underground storage organs and using the knowledge to improve such crops, the competitiveness of these types of storage organs can be strengthened. Starch is the most common storage compound in tubers and roots, but some crops accumulate compounds other than starch. This thesis examined representative underground storage organs accumulating starch, oil and sugars. These were: the oil-accumulating nutsedge (Cyperus esculentus), a half-grass which possesses the unusual ability to accumulate triacylglycerol in considerable levels in small tubers physiologically resembling those of potato; the sucrose-storing taproot of sugar beet (Beta vulgaris); and the starch- and sugar-storing taproot of parsnip (Pastinaca sativa). The lack of starch formation in sugar beet was examined in relation to expression of starch biosynthesis-related genes and enzymes in the taproot.

In parallel studies on potato (Solanum tuberosum), a classical starch accumulator, two different transgenic approaches to alter the metabolism and starch biosynthesis of tubers were tested. A novel finding was that expression of the oil transcription factor WRINKLED1 in potato tubers resulted in tubers accumulating oil and negatively affected starch biosynthesis. The oil-accumulating potato tubers shared structural similarities with young nutsedge. Assays on the transcriptome of sugar beet and parsnip revealed that transcripts of two plastidial genes responsible for energy import and phosphate hydrolysis were lower in sugar beet than in parsnip, indicating potential importance for starch accumulation. In potato, the importance of these two genes was assayed by silencing the genes. The outcome was potato tubers with severely affected starch biosynthesis, granule morphology, tuber yield, tuberisation and starch quality, confirming that the silenced genes play an important role in starch biosynthesis in potato tubers.

Authors/Creators:Turesson, Helle
Title:Carbon allocation in underground storage organs
Subtitle:studies on accumulation of starch, sugars and oil
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :2014
Depositing date:20 November 2014
Volume:2014:100
Number of Pages:53
Papers/manuscripts:
NumberReferences
I.Turesson, H., Marttila, S., Gustavsson, KE., Hofvander, P., Olsson, M., Bülow, L., Stymne, S., and Carlsson, A. S. (2010). Characterization of oil and starch accumulation in tubers of Cyperus esculentus var. sativus Cyperaceae. American Journal of Botany 97(11), 1884-1893.
II.Turesson, H., Andersson, M., Marttila, S., Thulin, I., and Hofvander, P. (2014). Starch biosynthetic genes and enzymes are expressed and active in sugar beet tap-root. BMC Plant Biology 14(104). DOI: 1471-2229/14/104
III.Hofvander, P., Turesson, H., Carlsson, A. S., Andersson, M. (2014). Expression of Arabidopsis WRINKLED1 in potato tubers lead to increased amounts of fatty acids in the form of triacylglycerols and membrane lipids while affecting tuber phenotype and starch composition. (Manuscript)
IV.Andersson M., Turesson, H., Fält., A, and Hofvander, P. (2014). Tuber-specific inhibition of inorganic pyrophosphatase and ATP/ADP translocator leads to major alterations in starch accumulation and tuber formation. (Manuscript)
Place of Publication:Alnarp
Publisher:Department of Plant Breeding, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8148-5
ISBN for electronic version:978-91-576-8149-2
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
Agrovoc terms:carbon, storage organs, starch, hexoses, oils, solanum tuberosum, beta vulgaris, cyperus esculentus, pastinaca sativa, genetically modified organisms, transcription factors
Keywords:carbon allocation, starch, sucrose, oil, hexose, Solanum tuberosum, Beta vulgaris, Cyperus esculentus, Pastinaca sativa, GMO, transcription factor
URN:NBN:urn:nbn:se:slu:epsilon-e-2235
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2235
ID Code:11664
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
External funders:FORMAS and Lyckeby Starch AB and Swedish Agency for Innovation Systems
Deposited By: Helle Turesson
Deposited On:24 Nov 2014 13:58
Metadata Last Modified:18 Dec 2014 07:33

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