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Metabolic engineering for production of complex lipids in tobacco (Nicotiana benthamiana) leaves and rice (Oryza sativa) endosperm

Aslan, Selcuk (2015). Metabolic engineering for production of complex lipids in tobacco (Nicotiana benthamiana) leaves and rice (Oryza sativa) endosperm. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2015:8
ISBN 978- 91-576-8214-7
eISBN 978-91-576-8215-4
[Doctoral thesis]

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Abstract

World chemical and energy supplies for industry are today highly dependent on unsustainable fossil reserves. Vegetable lipids with similar chemical structures to fossil oil could be the ultimate renewable solution to replace fossil oil and provide environmentally friendly feedstocks and energy resources. Wax esters and triacylglycerols (TAG) are two groups of lipid with applications in industry as lubricants, surfactants and biodiesel. However, global production of wax esters and TAG is limited to a few crop species such as jojoba, carnauba, oil palm, soybean and rapeseed. Further, there are restrictions on arable land for oilseed crop growth, and the demand for vegetable lipids is predicted to increase in coming decades. Therefore, there is an urgent need to establish sustainable technologies and develop new alternative oil crop species to meet an expected future vegetable lipid demand.

This thesis examined the potential for production of wax esters and TAG with different qualities in tobacco (Nicotiana benthamiana) leaves, and in rice (Oryza sativa) endosperm by metabolic engineering. In tobacco leaves, a new metabolic pathway was introduced into chloroplasts by combining bacterial- and plant-derived genes (AtFAR6, AtPES2, MaFAR, MhWS, tpMaFAR::MhWS, AtWRI1) for wax ester biosynthesis. Combinations of the gene functions resulted in production of wax esters with differing composition to a level of 0.9% of leaf dry weight (DW). The newly introduced pathway was further engineered by RNAi inhibition of the KASII gene. Additional co-expression of KASIIRNAi constructs resulted in increased palmitic/stearic acid ratio, which significantly improved wax ester production in AtFAR6-containing combinations. The novel gene fusion tpMaFAR::MhWS was further investigated by stable transformation of tobacco plants. Wax ester content in transformants was increased eight-fold compared with wild-type (WT). In rice, overexpression of both full-length and truncated Arabidopsis transcription factor AtWRI1 in endosperm tissues resulted respectively in sevenfold and four-fold accumulation of TAG content compared with WT. Taken together, these results showed that valuable oleochemicals could be increased considerably in plants.

Authors/Creators:Aslan, Selcuk
Title:Metabolic engineering for production of complex lipids in tobacco (Nicotiana benthamiana) leaves and rice (Oryza sativa) endosperm
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :21 January 2015
Depositing date:13 January 2015
Volume:2015:8
Number of Pages:76
Papers/manuscripts:
NumberReferences
I.Selcuk Aslan, Chuanxin Sun, Svetlana Leonova, Paresh Dutta, Peter Dörmann, Frédéric Domergue, Sten Stymne, Per Hofvander (2014). Wax esters of different compositions produced via engineering of leaf chloroplast metabolism in Nicotiana benthamiana. Metabolic Engineering 25, 103-112.
II.Selcuk Aslan, Per Hofvander, Paresh Dutta, Folke Sitbon, Chuanxin Sun (2014). Transient silencing of the β-ketoacyl-ACP synthase II genes is feasible in Nicotiana benthamiana for metabolic engineering of wax ester compositions. Submitted for publication.
III.Selcuk Aslan, Per Hofvander, Paresh Dutta, Chuanxin Sun, Folke Sitbon, (2014). Increased production of wax esters in transgenic tobacco plants by expression of a fatty acid reductase:wax synthase gene fusion. Submitted for publication.
IV.Yunkai Jin, Jun Su, Selcuk Aslan, Lu Jin, Margarita Aili, Chunlin Liu, Paresh Dutta, Per Hofvander, Folke Sitbon, Feng Wang, Chuanxin Sun (2014). Endosperm-specific expression of the Arabidopsis gene WRI1 in transgenic rice plants increases oil contents in the endosperm. Manuscript.
Place of Publication:Uppsala
Publisher:Department of Plant Biology, Swedish University of Agricultural Sciences
ISBN for printed version:978- 91-576-8214-7
ISBN for electronic version:978-91-576-8215-4
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
F Plant production > F60 Plant physiology and biochemistry
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
Agrovoc terms:nicotiana benthamiana, oryza sativa, waxes, esters, acylglycerols, lipids, leaves, endosperm, chloroplasts, genes, genetic engineering, tobacco, rice
Keywords:Wax ester, Triacylglycero, Nicotiana benthamiana (tobacco), Oryza sativa (rice), Chloroplast, Endosperm, Metabolic engineering
URN:NBN:urn:nbn:se:slu:epsilon-e-2301
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2301
ID Code:11739
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Plant Biology (from 140101)
External funders:FORMAS and Helge Ax:sson Johnsson Foundation
Deposited By: Selcuk Aslan
Deposited On:15 Jan 2015 07:47
Metadata Last Modified:28 Jan 2015 03:01

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