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New insights into carbon transport and incorporation to wood

Mahboubi, Amir (2015). New insights into carbon transport and incorporation to wood. Diss. (sammanfattning/summary) Umeå : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2015:3
ISBN 978-91-576-8204-8
eISBN 978-91-576-8205-5
[Doctoral thesis]

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Abstract

Wood formation in trees requires carbon import from the photosynthetic tissues. In several tree species the majority of this carbon is derived from sucrose transported in the phloem. This thesis describes experimental work on the mechanism of radial sucrose transport from phloem to developing wood and subsequent incorporation of carbon into wood polymers.

I investigated the role of active sucrose transport during secondary cell wall formation in hybrid aspen (Populus tremula x tremuloides). Reduction of a plasma membrane localised sucrose transporter (PttSUT3) decreased carbon allocation to secondary walls of wood fibers. The results show the importance of active sucrose transport for wood formation in a symplasmic phloem loading tree species, and identify PttSUT3 as a principal transporter for carbon delivery into secondary cell wall forming wood fibers.

To investigate the temporal dynamics of carbon transport and wood polymer biosynthesis, I labelled two-month-old hybrid aspen trees with ¹³CO₂ and tracked the flux of ¹³C from leaves to developing wood. Analysis of the cell wall polymer labelling patterns using 2D-NMR revealed temporal differences in the labelling of carbohydrates and lignin subunits. Further analysis showed that ¹³C incorporation into different wood polymers is influenced by the diurnal cycle.

Primary metabolism enzymes in the developing wood play an important role in carbon partitioning to wood cell wall polymers. In this part of the study, the activity of eight primary metabolism enzymes linking sucrose to cell wall precursor biosynthesis was determined in phloem, cambium and in different stages of wood development. Comparison of enzyme activity measurements with transcript and metabolite profiles across the developing wood suggested a central role for transcriptional regulation of carbon allocation to wood.

Combined, the results of the three projects provide new insights into the mechanism and regulation of carbon allocation to developing wood.

Authors/Creators:Mahboubi, Amir
Title:New insights into carbon transport and incorporation to wood
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :15 January 2015
Volume:2015:3
Number of Pages:62
Papers/manuscripts:
NumberReferences
I.Amir Mahboubi, Christine Ratke, Andras Gorzas, Manoj Kumar, Ewa J. Mellerowicz, and Totte Niittylä (2013). Aspen SUCROSE TRANSPORTER3 Allocates Carbon into Wood Fibers. Plant Physiology 163 (4), 1729–1740.
II.Amir Mahboubi*, Pernilla Linden*, Mattias Hedenström, Thomas Moritz and Totte Niittylä (2014). Carbon-13 tracking after ¹³CO₂ supply revealed diurnal patterns of wood biosynthesis in aspen. Manuscript.
III.Melissa Roach, Stephanie Arrivault, Amir Mahboubi, Nicole Krohn, Ronan Sulpice, Mark Stitt and Totte Niittylä (2014). Spatial analysis of primary metabolism in developing wood of aspen suggested a central role for transcriptional regulation in carbon allocation to wood. Manuscript.
Place of Publication:Umeå
Publisher:Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8204-8
ISBN for electronic version:978-91-576-8205-5
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F60 Plant physiology and biochemistry
K Forestry > K01 Forestry - General aspects
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
Agrovoc terms:populus tremula, populus tremuloides, hybrids, wood, lignification, carbon dioxide, nutrient transport, sucrose, cell walls, wood anatomy, genetics
Keywords:Carbon transport, Carbon incorporation, Wood
URN:NBN:urn:nbn:se:slu:epsilon-e-2302
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2302
ID Code:11744
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: Mir Amir Hossein Mahboubi
Deposited On:15 Jan 2015 13:10
Metadata Last Modified:15 Jan 2015 13:10

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