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Structural and interaction studies of polysaccharides by NMR spectroscopy

Lundqvist, Lena (2015). Structural and interaction studies of polysaccharides by NMR spectroscopy. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2015:17
ISBN 978-91-576-8232-1
eISBN 978-91-576-8233-8
[Doctoral thesis]

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Abstract

In this thesis, polysaccharides of different origins have been investigated by NMR spectroscopy with regard to structures and interactions.

In paper I, hydrogen bonding interactions in kappa- (κ) and kappa/mu- (κ/μ) hybrid carrageenan oligosaccharides were studied by NMR spectroscopy of hydroxy protons. Weak hydrogen bonding over the 1→4 glycosidic linkage of μ-carrabiose was observed in the κ/μ oligosaccharides. In the hexasaccharide κμκ and octasaccharide κμμκ, one and two hydrogen bonds respectively were found over the 1→4 glycosidic linkage. In the decasaccharide κμμμκ, a loss of a hydrogen bond in the inner μ-unit was observed,
suggesting higher flexibility in a μ-unit having other μ-units as neighbors.

In papers II and III, the activities and substrate
specificities of three new alginate lyases were characterized. The alginate lyase from the marine bacteria Pseudomonas alginovora showed an endo-cleaving activity with a strict specificity for M-block alginates. The AlyA1 lyase from the marine bacteria Zobellia galactanivorans, showed an endo-cleaving activity with a strict specificity for G-block alginates, whereas AlyA5 showed an exo-cleaving activity with a broad substrate tolerance, degrading oligosaccharides down to monosaccharides.

Paper IV describes the elucidation of the primary structure of the O-specific polysaccharide and core oligosaccharide from the lipopolysaccharide produced by the Gram-negative bacteria Plesiomonas shigelloides strain CNCTC 92/89 (O24:H8). The O-specific polysaccharide was composed of a tetrasaccharide repeating unit: →3) α FucpNAc-(1→3)-α-GalpNAcA-(1→3)-α-QuipNAc-(1→, with α-RhapNAc linked (1→4) to α-GalpNAcA. The core oligosaccharide was composed of a decasaccharide.

Authors/Creators:Lundqvist, Lena
Title:Structural and interaction studies of polysaccharides by NMR spectroscopy
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :2015
Depositing date:2015
Volume:2015:17
Number of Pages:75
Papers/manuscripts:
NumberReferences
I.Eric Morssing Vilén, Lena C. E. Lundqvist, Diane Jouanneau, William Helbert, and Corine Sandström (2010). NMR Study on Hydroxy Protons of κ- and κ-/µ-Hybrid Carrageenan Oligosaccharides: Experimental Evidence of Hydrogen Bonding and Chemical Exchange Interactions in κ/µ Oligosaccharides. Biomacromolecules 11, 3487–3494.
II.Lena C. E. Lundqvist, Murielle Jam, Tristan Barbeyron, Mirjam Czjzek and Corine Sandström (2012). Substrate Specificity of the Recombinant Alginate Lyase from the Marine Bacteria Pseudomonas alginovora. Carbohydrate Research 352, 44-50.
III.François Thomas, Lena C. E. Lundqvist, Murielle Jam, Alexandra Jeudy, Tristan Barbeyron, Corine Sandström, and Mirjam Czjzek (2013). Comparative Characterization of Two Marine Alginate Lyases from Zobellia galactanivorans Reveals Distinct Modes of Action and Exquisite Adaptation to Their Natural Substrate. The Journal of Biological Chemistry 288 (32), 23021-23037.
IV.Lena C. E. Lundqvist, Marta Kaszowska, and Corine Sandström. Structural Study of the O-specific Polysaccharide and the Core Oligosaccharide from the Lipopolysaccharide Produced by Plesiomonas shigelloides O24:H8 (Strain CNCTC 92/89) (manuscript).
Place of Publication:Uppsala
Publisher:Institutionen för kemi och bioteknologi, Sveriges lantbruksuniversitet
ISBN for printed version:978-91-576-8232-1
ISBN for electronic version:978-91-576-8233-8
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:X Agricola extesions > X50 Chemistry
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 104 Chemical Sciences > Analytical Chemistry
(A) Swedish standard research categories 2011 > 1 Natural sciences > 104 Chemical Sciences > Inorganic Chemistry
Agrovoc terms:carbohydrates, polysaccharides, oligosaccharides, chemical structure, chemicophysical properties, alginates, lyases, carrageenans, plesiomonas shigelloides, nmr spectroscopy
Keywords:NMR, Polysaccharide, Carbohydrate, Oligosaccharide, Hydroxy proton, Lipopolysaccharide, Plesiomonas shigelloides
URN:NBN:urn:nbn:se:slu:epsilon-e-2318
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2318
ID Code:11754
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Chemistry and Biotechnology (140101-161231)
External funders:Carl Tryggers stiftelse för vetenskaplig forskning and EU FP7
Deposited By: Lena C. E. Lundqvist
Deposited On:26 Jan 2015 10:34
Metadata Last Modified:15 Feb 2015 02:08
Project info:
Name:Novel polysaccharide modifying enzymes to optimise the potential of hydrocolloides for food and medical applications
Acronym:POLY MOD E
ID:222628
Programme:FP7

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