Niu, Jinzhi and Cappelle, Kaat and Rodrigues De Miranda, Joachim and Smagghe, Guy and Meeus, Ivan
(2014).
Analysis of reference gene stability after Israeli acute paralysis virus infection in bumblebees Bombus terrestris.
Journal of invertebrate pathology. 115
, 76-79
[Research article]
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Official URL: http://dx.doi.org/10.1016/j.jip.2013.10.011
Abstract
To date, there are no validated internal reference genes for the normalization of RT-qPCR data from virus infection experiments with pollinating insects. In this study we evaluated the stability of five candidate internal reference genes: elongation factor-1 -alpha (ELF1 alpha), peptidylprolyl isomerase A (PPIA), 60S ribosomal protein L23 (RPL23), TATA-binding protein (TBP) and polyubiquitin (UBI), in relation to Israeli acute paralysis virus (IAPV) infection of Bombus terrestris. We investigated the stability of these genes: in whole bodies and individual body parts, as well as in whole bodies collected at different time intervals after infection with IAPV. Our data identified PPIA as the single, most-optimal internal reference gene and the combination of PPAI-RPL23-UBI as a fully-sufficient multiple internal reference genes set for IAPV infection experiments in B. terrestris.
| Authors/Creators: | Niu, Jinzhi and Cappelle, Kaat and Rodrigues De Miranda, Joachim and Smagghe, Guy and Meeus, Ivan | ||||
|---|---|---|---|---|---|
| Title: | Analysis of reference gene stability after Israeli acute paralysis virus infection in bumblebees Bombus terrestris | ||||
| Series Name/Journal: | Journal of invertebrate pathology | ||||
| Year of publishing : | 2014 | ||||
| Volume: | 115 | ||||
| Page range: | 76-79 | ||||
| Number of Pages: | 4 | ||||
| Publisher: | Elsevier | ||||
| ISSN: | 1096-0805 | ||||
| Language: | English | ||||
| Publication Type: | Research article | ||||
| Refereed: | Yes | ||||
| Article category: | Scientific peer reviewed | ||||
| Version: | Accepted version | ||||
| Full Text Status: | Public | ||||
| Agris subject categories.: | X Agricola extesions > X50 Chemistry | ||||
| Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology | ||||
| Agrovoc terms: | gene expression, viruses, bombus terrestris | ||||
| Keywords: | gene expression, bee virus, RT-qPCR, bicistroviruses, qBase(PLUS) | ||||
| URN:NBN: | urn:nbn:se:slu:epsilon-e-2809 | ||||
| Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2809 | ||||
| Additional ID: |
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| ID Code: | 11854 | ||||
| Faculty: | NJ - Fakulteten för naturresurser och jordbruksvetenskap | ||||
| Department: | (NL, NJ) > Dept. of Ecology (S) > Dept. of Ecology | ||||
| Deposited By: | SLUpub Connector | ||||
| Deposited On: | 29 Jul 2015 08:51 | ||||
| Metadata Last Modified: | 09 Sep 2020 14:17 |
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