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Analysis of reference gene stability after Israeli acute paralysis virus infection in bumblebees Bombus terrestris

Niu, Jinzhi and Cappelle, Kaat and Rodrigues De Miranda, Joachim and Smagghe, Guy and Meeus, Ivan (2014). Analysis of reference gene stability after Israeli acute paralysis virus infection in bumblebees Bombus terrestris. Journal of invertebrate pathology. 115, 76-79
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Official URL: http://dx.doi.org/10.1016/j.jip.2013.10.011

Abstract

To date, there are no validated internal reference genes for the normalization of RT-qPCR data from virus infection experiments with pollinating insects. In this study we evaluated the stability of five candidate internal reference genes: elongation factor-1 -alpha (ELF1 alpha), peptidylprolyl isomerase A (PPIA), 60S ribosomal protein L23 (RPL23), TATA-binding protein (TBP) and polyubiquitin (UBI), in relation to Israeli acute paralysis virus (IAPV) infection of Bombus terrestris. We investigated the stability of these genes: in whole bodies and individual body parts, as well as in whole bodies collected at different time intervals after infection with IAPV. Our data identified PPIA as the single, most-optimal internal reference gene and the combination of PPAI-RPL23-UBI as a fully-sufficient multiple internal reference genes set for IAPV infection experiments in B. terrestris.

Authors/Creators:Niu, Jinzhi and Cappelle, Kaat and Rodrigues De Miranda, Joachim and Smagghe, Guy and Meeus, Ivan
Title:Analysis of reference gene stability after Israeli acute paralysis virus infection in bumblebees Bombus terrestris
Series/Journal:Journal of invertebrate pathology (1096-0805)
Year of publishing :2014
Volume:115
Page range:76-79
Number of Pages:4
Publisher:Elsevier
ISSN:1096-0805
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Accepted version
Full Text Status:Public
Agris subject categories.:X Agricola extesions > X50 Chemistry
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
Agrovoc terms:gene expression, viruses, bombus terrestris
Keywords:gene expression, bee virus, RT-qPCR, bicistroviruses, qBase(PLUS)
URN:NBN:urn:nbn:se:slu:epsilon-e-2809
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2809
Additional ID:
Type of IDID
Web of Science (WoS)000329558800012
ID Code:11854
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Ecology
(S) > Dept. of Ecology
Deposited By: SLUpub Connector
Deposited On:29 Jul 2015 08:51
Metadata Last Modified:15 Dec 2015 09:10

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