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Effect of oral infection with Kashmir bee virus and Israeli acute paralysis virus on bumblebee (Bombus terrestris) reproductive success

Meeus, Ivan and Rodrigues De Miranda, Joachim and de Graaf, Dirk C. and Wäckers, Felix and Smagghe, Guy (2014). Effect of oral infection with Kashmir bee virus and Israeli acute paralysis virus on bumblebee (Bombus terrestris) reproductive success. Journal of invertebrate pathology. 121, 64-69
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Official URL: http://dx.doi.org/10.1016/j.jip.2014.06.011

Abstract

Israeli acute paralysis virus (IAPV) together with Acute bee paralysis virus (ABPV) and Kashmir bee virus (KBV) constitute a complex of closely related dicistroviruses. They are infamous for their high mortality after injection in honeybees. These viruses have also been reported in non-Apis hymenopteran pollinators such as bumblebees, which got infected with IAPV when placed in the same greenhouse with IAPV infected honeybee hives. Here we orally infected Bombus terrestris workers with different doses of either IAPV or KBV viral particles. The success of the infection was established by analysis of the bumblebees after the impact studies: 50 days after infection. Doses of 0.5 x 10(7) and 1 x 10(7) virus particles per bee were infectious over this period, for IAPV and KBV respectively, while a dose of 0.5 x 10(6) IAPV particles per bee was not infectious. The impact of virus infection was studied in micro-colonies consisting of 5 bumblebees, one of which becomes a pseudo-queen which proceeds to lay unfertilized (drone) eggs. The impact parameters studied were: the establishment of a laying pseudo-queen, the timing of egg-laying, the number of drones produced, the weight of these drones and worker mortality. In this setup KBV infection resulted in a significant slower colony startup and offspring production, while only the latter can be reported for IAPV. Neither virus increased worker mortality, at the oral doses used. We recommend further studies on how these viruses transmit between different pollinator species. It is also vital to understand how viral prevalence can affect wild bee populations because disturbance of the natural host-virus association may deteriorate the already critically endangered status of many bumblebee species.

Authors/Creators:Meeus, Ivan and Rodrigues De Miranda, Joachim and de Graaf, Dirk C. and Wäckers, Felix and Smagghe, Guy
Title:Effect of oral infection with Kashmir bee virus and Israeli acute paralysis virus on bumblebee (Bombus terrestris) reproductive success
Series/Journal:Journal of invertebrate pathology (1096-0805)
Year of publishing :2014
Depositing date:10 February 2015
Volume:121
Page range:64-69
Number of Pages:6
Publisher:Elsevier
ISSN:1096-0805
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Accepted version
Full Text Status:Public
Agris subject categories.:X Agricola extesions > X30 Life sciences
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Other Biological Topics
Agrovoc terms:viruses, bombus, pathogens
Keywords:Israeli acute paralysis virus, Kashmir bee virus, dicistroviridae, bumblebees, multi-host pathogens
URN:NBN:urn:nbn:se:slu:epsilon-e-2397
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2397
Additional ID:
Type of IDID
Web of Science (WoS)000341284400010
DOI10.1016/j.jip.2014.06.011
ID Code:11855
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Ecology
(S) > Dept. of Ecology
Deposited By: SLUpub Connector
Deposited On:06 Aug 2015 11:25
Metadata Last Modified:26 Feb 2016 07:27

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