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Host-pathogen interactions in root infecting oomycete species

Hadji Mollahossein, Sara (2015). Host-pathogen interactions in root infecting oomycete species. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2015:9
ISBN 978-91-576-8216-1
eISBN 978-91-576-8217-8
[Doctoral thesis]

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Abstract

The oomycetes include some of the most devastating pathogens on both cultivated crops and wild plants. In the genus Phytophthora some closely related species have a broad host range, while others are very host specific. The aim of this project was to gain an understanding of the mechanisms underlying the differentiation of a subgroup of root-infecting Phytophthora species and to gain knowledge about the plant immune responses triggered by distantly related oomycetes that adapted to the same legume host.

We investigated the zoospore chemotaxis of legume-root infecting Phytophthora species to different isoflavonoid compounds and explored a possible connection to host preference. Our results showed that specific chemotaxis towards host isoflavones is of limited importance in Phytophthora sojae and Phytophthora vignae, while, specific chemotaxis of Phytophthora pisi and Phytophthora niederhauserii indicated an adaptation to their pathogenicity on the host and lack of pathogenicity on non-host plants.

The comparative proteomic study of P. pisi and P. sojae in germinating cysts and hyphae, using tandem mass spectrometry, revealed specific and common pathogenicity factors involved in initiation of infection and host specificity such as serine proteases, membrane transporters and ricin-B lectin in these closely related species. Furthermore, the data suggested that germinating cysts catabolize lipid reserves through the β-oxidation pathway and the glyoxylate cycle to initiate infection.

The transcriptomic response of pea plants towards Aphanomyces euteiches and P. pisi, two distantly related oomycetes, was studied during early phase of infection, using a microarray approach. The results deciphered common and specific immune mechanisms towards these pathogens. Activation of cell wall modification, regulation of jasmonic acid biosynthesis and induction of the ethylene signaling pathway were among the common transcriptional responses to both pathogens. However, induction of chalcone synthesis and auxin signaling were specific transcriptional changes against A. euteiches.

Authors/Creators:Hadji Mollahossein, Sara
Title:Host-pathogen interactions in root infecting oomycete species
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :February 2015
Depositing date:25 February 2015
Volume:2015:9
Number of Pages:68
Papers/manuscripts:
NumberReferences
IHosseini, S., Heyman, F., Olsson, U., Broberg, A., Jensen Funck, D. and Karlsson, M., (2014). Zoospore chemotaxis of closely related legume-root infecting Phytophthora species towards host isoflavones. Plant Pathology 63(3), 708-7014.
IIHosseini, S., Resjö, S., Liu, Y., Brandström-Durling, M., Heyman, F., Levander, F., Liu, Y., Elfstrand, M., Funck Jensen, D., Andreasson, E. and Karlsson, M., (2015). Comparative proteomic analysis of hyphae and germinating cysts of Phytophthora pisi and Phytophthora sojae. Journal of Proteomics 117, 24-40.
IIIHosseini, S., Elfstrand, M., Heyman, F., Funck Jensen, D. and Karlsson, M., (2015). Deciphering common and specific transcriptional immune responses in pea towards the oomycete pathogens Aphanomyces euteiches and Phytophthora pisi. (Submitted).
Place of Publication:Uppsala
Publisher:Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8216-1
ISBN for electronic version:978-91-576-8217-8
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
H Protection of plants and stored products > H20 Plant diseases
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Agrovoc terms:phytophthora, pathogens, oomycetes, fungal diseases, plants, immune response, genes, pathogenicity, inoculation, chemotaxis, host pathogen relations
Keywords:Oomycete, Phytophthora, Proteomics, Plant immunity
URN:NBN:urn:nbn:se:slu:epsilon-e-2464
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2464
ID Code:11945
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: Sara Hosseini
Deposited On:26 Feb 2015 09:59
Metadata Last Modified:17 Sep 2015 08:30

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