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The role of nucleoside diphosphate kinase in plant mitochondria

Johansson, Monika (2006). The role of nucleoside diphosphate kinase in plant mitochondria. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2006:74
ISBN 91-576-7123-0
[Doctoral thesis]

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Abstract

Nucleoside Diphosphate Kinase (NDPK) catalyses the transfer of a phosphate from nucleoside triphosphates to a nucleoside diphosphate, is ubiquitously found in all organisms from bacteria to human. It was discovered that the genes nm23 and awd, which encode NDPKs are involved in tumour metastasis and Drosophila development, respectively. Thus, NDPK isoforms have been suggested to have specific regulatory functions in addition to their catalytic activity. Plant NDPKs are also involved in a number of intracellular signalling events such as phytochrome A response, UV-B light signalling and heat shock response. The main focus in this thesis concerns the role of the plant (Pisum sativum L. cv Oregon sugarpod) mitochondrial NDPK3 isoform.

The NDPK3 is localized to both the intermembrane space and to the mitochondrial inner membrane. The membrane bound NDPK3 is firmly attached to the membrane through the ATP/ADP translocase. The ATP/ADP translocase and NDPK3 complex may be a part of the contact sites for channelling metabolites from mitochondria to cytosol and vice versa.

NDPK3 was shown to be dually targeted to both mitochondria and chloroplasts where the major amount of the protein is found in mitochondria. The protein was crystallized and the first X-ray structure of a plant NDPK is reported. In agreement with other eukaryotic NDPKs, the plant enzyme is a hexamer. Two conserved serine residues, S119 and S69 involved in serine autophosphorylation and oligomerization, respectively, was identified. We show that the Ser autophosphorylation depends on enzyme activity. The mutation of S69 to Ala decreased the enzymatic activity dramatically. Changes in the oligomeric pattern of S69A were observed. Thus, the S69 residue is important for the stabilization of the oligomeric state of NDPK3.

Adenylate Kinase was identified as an interacting partner of the IMS located NDPK3. The interaction modulates the activity of the enzymes where Adenylate Kinase stimulates NDPK3 and NDPK3 inhibits Adenylate Kinase with unchanged ADP production as an outcome. Cyclic AMP (cAMP) and calcium inhibit the activity of both NDPK3 and Adenylate Kinase. This is a novel regulatory relationship between cAMP and calcium signalling and nucleotide metabolism mediated by NDPK3 and Adenylate Kinase and their interaction.

Authors/Creators:Johansson, Monika
Title:The role of nucleoside diphosphate kinase in plant mitochondria
Series/Journal:Acta Universitatis agriculturae Sueciae
Year of publishing :2006
Volume:2006:74
Page range:1-40
Number of Pages:40
Papers/manuscripts:
NumberReferences
I.Knorpp, C., Johansson, M. & Baird, A. M. 2003. Plant mitochondrial nucleoside diphosphate kinase is attached to the membrane through interaction with the adenine nucleotide translocator. FEBS Letters 555, 363-366.
II.Johansson, M., Mackenzie-Hose, A., Andersson, I. & Knorpp, C. 2004. Structure and mutational analysis of a plant mitochondrial nucleoside diphosphate kinase. Identification of residues involved in serine phosphorylation and oligomerization. Plant Physiology 136, 3034-3042.
III.Hammargren, J., Sundström, J., Johansson, M., Bergman, P. & Knorpp, C. 2006. On the phylogeny, expression and targeting of plant Nucleoside Diphosphate Kinases. Physiologia Plantarum Published online 23 August 2006. PPL 0794.
IV.Johansson, M., Uppsäll, E., Mackenzie, A. & Knorpp, C. 2006. The activity of Nucleoside Diphosphate Kinase and Adenylate Kinase are influenced by cAMP and calcium, as well as by their interaction. (Submitted).
Place of Publication:Uppsala
Publisher:Dept. of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences
ISBN for printed version:91-576-7123-0
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
F Plant production > F60 Plant physiology and biochemistry
Agrovoc terms:proteins, protein kinase, mitochondria, atp, adp, amp, calcium
Keywords:NDPK, plant mitochondria Adenylate Kinase, ATP/ADP translocase, X-ray structure, cAMP, calcium
URN:NBN:urn:nbn:se:slu:epsilon-e-1620
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-1620
ID Code:1201
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: Monika Johansson
Deposited On:04 Sep 2013 20:23
Metadata Last Modified:13 Dec 2014 12:04

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