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Assessment of diversity in Harpagophytum with RAPD and ISSR markers provides evidence of introgression

Muzila, Mbaki and Werlemark, Gun and Ortiz Rios, Rodomiro Octavio and Sehic, Jasna and Fatih, Moneim and Setshogo, Moffat and Mpoloka, Wata and Nybom, Hilde (2014). Assessment of diversity in Harpagophytum with RAPD and ISSR markers provides evidence of introgression. Hereditas. 151:4-5, 91-101
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Abstract

The genus Harpagophytumhas two species: H. procumben swhich is an important medicinal plant in southern Africa, and H. zeyheri. Genetic diversity in 96 samples, obtained by germinating seeds collected from Botswana, was assessed using six inter-simple sequence repeat (ISSR) and 10 random amplified polymorphic DNA (RAPD) primers. These DNA markers yielded a total of 138 polymorphic bands. Polymorphism information content (PIC) ranged from 0.06 to 0.39 for ISSR primers, and from 0.09 to 0.43 for RAPD primers. Jaccard's similarity coefficients were highest when seedlings derived from the same fruit capsule were compared, while seedlings from different fruits on the same plant had intermediate values. The lowest values were recorded among seedlings from different plants. These results were consistent with an outcrossing breeding system in Harpagophytum. Analysis of molecular variance revealed significant differentiation (P < 0.01) between taxonomic units within Harpagophytum. About 39% of the variability occurred between the two species, H. procumbens and H. zeyheri. Plants with an intermediate morphology, i.e. putative hybrids (PH), showed 21% differentiation when compared with H. procumbens ssp. procumbens (PP), and 19% when compared with H. procumbens ssp. transvaalense (PT) or with H. zeyheri (ZZ). In addition, a deviating variant of PT was identified, here termed ‘procumbens new variety’ (PN). PN showed only 9% differentiation when compared with PT, 22% when compared with PP or with PH, and 41% when compared with ZZ. Considerable differentiation between the two Harpagophytum species was revealed also by a cluster analysis. Introgression was, however, suggested by the intermediate position of the putative hybrid plants in a principal component analysis while inter-specific gene flow was shown by a Bayesian genetic structure analysis.

Authors/Creators:Muzila, Mbaki and Werlemark, Gun and Ortiz Rios, Rodomiro Octavio and Sehic, Jasna and Fatih, Moneim and Setshogo, Moffat and Mpoloka, Wata and Nybom, Hilde
Title:Assessment of diversity in Harpagophytum with RAPD and ISSR markers provides evidence of introgression
Series/Journal:Hereditas (1601-5223)
Year of publishing :2014
Volume:151
Number:4-5
Page range:91-101
Number of Pages:11
Publisher:Mendeleian Society of Lund
Associated Programs and Other Stakeholders:Z - SLU - Libray > Odla mera
ISSN:1601-5223
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Horticulture
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
Keywords:Harpagophytum, diversity, ISSR, RAPD, genetics
URN:NBN:urn:nbn:se:slu:epsilon-e-2580
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2580
Additional ID:
Type of IDID
Web of Science (WoS)000344650000004
DOI10.1111/hrd2.00052
ID Code:12084
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:21 Apr 2015 09:17
Metadata Last Modified:17 Feb 2016 05:52

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