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Exogenous estradiol improves shell strength in laying hens at the end of the laying period

Wistedt, Anna and Ridderstråle, Yvonne and Wall, Helena and Holm, Lena (2014). Exogenous estradiol improves shell strength in laying hens at the end of the laying period. Acta veterinaria Scandinavica. 56:34, 1-11
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Official URL: http://dx.doi.org/10.1186/1751-0147-56-34

Abstract

Background: Cracked shells, due to age related reduction of shell quality, are a costly problem for the industry. Parallel to reduced shell quality the skeleton becomes brittle resulting in bone fractures. Calcium, a main prerequisite for both eggshell and bone, is regulated by estrogen in a complex manner. The effects of estrogen, given in a low continuous dose, were studied regarding factors involved in age related changes in shell quality and bone strength of laying hens. A pellet containing 0.385 mg estradiol 3-benzoate (21-day-release) or placebo was inserted subcutaneously in 20 birds each of Lohmann Selected Leghorn (LSL) and Lohmann Brown (LB) at 70 weeks of age. Eggs were collected before and during the experiment for shell quality measurements. Blood samples for analysis of total calcium were taken three days after the insertion and at sacrifice (72 weeks). Right femur was used for bone strength measurements and tissue samples from duodenum and shell gland were processed for morphology, immunohistochemical localization of estrogen receptors (ER alpha, ER beta), plasma membrane calcium ATPase (PMCA) and histochemical localization of carbonic anhydrase (CA). Results: Estrogen treatment increased shell thickness of both hybrids. In addition, shell weight and shell deformation improved in eggs from the brown hybrids. The more pronounced effect on eggs from the brown hybrid may be due to a change in sensitivity to estrogen, especially in surface epithelial cells of the shell gland, shown as an altered ratio between ER alpha and ER beta. A regulatory effect of estrogen on CA activity, but not PMCA, was seen in both duodenum and shell gland, and a possible connection to shell quality is discussed. Bone strength was unaffected by treatment, but femur was stronger in LSL birds suggesting that the hybrids differ in calcium allocation between shell and bone at the end of the laying period. Plasma calcium concentrations and egg production were unaffected.
Conclusions: A low continuous dose of estrogen improves shell strength but not bone strength in laying hens at the end of the laying period.

Authors/Creators:Wistedt, Anna and Ridderstråle, Yvonne and Wall, Helena and Holm, Lena
Title:Exogenous estradiol improves shell strength in laying hens at the end of the laying period
Series/Journal:Acta veterinaria Scandinavica (0044-605X)
Year of publishing :2014
Volume:56
Number:34
Page range:1-11
Number of Pages:11
Publisher:BioMed Central
ISSN:0044-605X
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:L Animal production > L70 Veterinary science and hygiene - General aspects
Subjects:Obsolete subject words > VETERINARY MEDICINE > Other veterinary medicine
Agrovoc terms:estradiol, oogenesis, hens
Keywords:Exogenous estradiol, Eggshell formation, Carbonic anhydrase, Estrogen receptors, Bone strength, Eggshell quality, Domestic hen
URN:NBN:urn:nbn:se:slu:epsilon-e-2615
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2615
Additional ID:
Type of IDID
Web of Science (WoS)000338452700001
ID Code:12106
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Anatomy, Physiology and Biochemistry
(VH) > Dept. of Animal Nutrition and Management
Deposited By: SLUpub Connector
Deposited On:05 May 2015 06:56
Metadata Last Modified:12 Feb 2016 04:00

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