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Regulation of embryo development in Norway spruce by WOX transcription factors

Zhu, Tianqing (2015). Regulation of embryo development in Norway spruce by WOX transcription factors. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2015:34
ISBN 978-91-576-8266-6
eISBN 978-91-576-8267-3
[Doctoral thesis]

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Abstract

In seed plants, the apical-basal axis of the plant body is established during early embryogenesis. Major regulatory genes of the apical-basal axis formation belong to the WUSCHEL-RELATED HOMEOBOX (WOX) gene family of transcription factors. The spatiotemporal expression pattern and the molecular role of the WOX genes has mainly been studied in the angiosperm model plant Arabidopsis (Arabidopsis thaliana). Similar information in conifers is limited. The aim of my thesis has been to characterize WOX genes in Norway spruce (Picea abies) and to elucidate the function of WOX genes expressed during embryo development.

We cloned 11 WOX homologs from Norway spruce and examined their phylogenetic relationship to WOX genes from other species. The phylogenetic analyses showed that the major diversification within the WOX gene family took place before the gymnosperm-angiosperm split. PaWOX8/9, PaWOX2 and PaWOX3, which are expressed in embryos, were selected for further studies.

PaWOX8/9 and PaWOX2 are highly expressed in early and late embryos, and PaWOX3 is highly expressed in mature embryos. Functional studies were performed in RNAi lines where the genes were down-regulated. Embryos in PaWOX8/9 RNAi lines showed a disturbed apical-basal patterning caused by abnormal orientation of the cell division plane at the basal part of the embryonal mass. In PaWOX2 RNAi lines, vacuolated cells differentiated on the surface of the embryonal mass and the embryos failed to form a proper protoderm. Down-regulation of PaWOX3 disturbed lateral margin outgrowth in cotyledons and needles.

Taken together, our results indicate that WOX8/9, WOX2 and WOX3 exert evolutionarily conserved functions during embryo development. We can therefore conclude that the regulatory networks of embryo development are at least partly conserved between angiosperms and gymnosperms.

Authors/Creators:Zhu, Tianqing
Title:Regulation of embryo development in Norway spruce by WOX transcription factors
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :2015
Depositing date:2015
Volume:2015:34
Number of Pages:52
Papers/manuscripts:
NumberReferences
IHedman, H.*, Zhu, T.*, von Arnold, S., and Solhlberg, J. J. (2013). Analysis of the WUSCHEL-RELATED HOMEOBOX gene family in the conifer Picea abies reveals extensive conservation as well as dynamic patterns. BMC plant Biol BMC Plant Biol, 13, p. 89. * the first two authors contributed equally.
IIZhu, T., Moschou, P.N., Alvarez, J.M., Sohlberg, J.J. and von Arnold, S. (2014). WUSCHEL-RELATED HOMEOBOX 8/9 is important for proper embryo patterning in the gymnosperm Norway spruce. J Exp Bot 65(22), 6543-6552.
IIIZhu, T., Moschou, P.N., Alvaréz, J.M., Sohlberg, J.J. and von Arnold, S. 2014. WUSCHEL-RELATED HOMEOBOX 2 is important for protoderm development in the gymnosperm Norway spruce. (Manuscript).
IVAlvaréz, J.M., Sohlberg, J.J., Engstöm, P., Zhu, T., Englund, M., Moschou, P.N. and von Arnold, S. WUSCHEL-RELATED HOMEOBOX 3 regulates lateral organ formation in shoots in Norway spruce. (Submitted).
Place of Publication:Uppsala
Publisher:Department of Plant Biology, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8266-6
ISBN for electronic version:978-91-576-8267-3
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
F Plant production > F62 Plant physiology - Growth and development
K Forestry > K01 Forestry - General aspects
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
Agrovoc terms:picea abies, embryonic development, homeoboxes, transcription factors, genetic variation, phylogeny
Keywords:apical-basal polarization, conifers, Norway spruce, phylogeny, somatic embryogenesis, stem cells, WUSCHEL-RELATED HOMEOBOX genes
URN:NBN:urn:nbn:se:slu:epsilon-e-2591
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2591
ID Code:12139
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Plant Biology (from 140101)
Deposited By: Tianqing Zhu
Deposited On:23 Apr 2015 12:00
Metadata Last Modified:21 May 2015 17:57

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