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Fragmentation of tRNA in Phytophthora infestans asexual life cycle stages and during host plant infection

Åsman, Anna and Vetukuri, Ramesh and Jahan, Sultana and Fogelqvist, Johan and Corcoran, Pádraic and Avrova, Anna and Whisson, Stephen and Dixelius, Christina (2014). Fragmentation of tRNA in Phytophthora infestans asexual life cycle stages and during host plant infection. BMC Microbiology. 14, 1-12
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Official URL: http://dx.doi.org/10.1186/s12866-014-0308-1

Abstract

The oomycete Phytophthora infestans possesses active RNA silencing pathways, which presumably enable this plant pathogen to control the large numbers of transposable elements present in its 240 Mb genome. Small RNAs (sRNAs), central molecules in RNA silencing, are known to also play key roles in this organism, notably in regulation of critical effector genes needed for infection of its potato host.Results: To identify additional classes of sRNAs in oomycetes, we mapped deep sequencing reads to transfer RNAs (tRNAs) thereby revealing the presence of 19-40 nt tRNA-derived RNA fragments (tRFs). Northern blot analysis identified abundant tRFs corresponding to half tRNA molecules. Some tRFs accumulated differentially during infection, as seen by examining sRNAs sequenced from P. infestans-potato interaction libraries. The putative connection between tRF biogenesis and the canonical RNA silencing pathways was investigated by employing hairpin RNA-mediated RNAi to silence the genes encoding P. infestans Argonaute (PiAgo) and Dicer (PiDcl) endoribonucleases. By sRNA sequencing we show that tRF accumulation is PiDcl1-independent, while Northern hybridizations detected reduced levels of specific tRNA-derived species in the PiAgo1 knockdown line.Conclusions: Our findings extend the sRNA diversity in oomycetes to include fragments derived from non-protein-coding RNA transcripts and identify tRFs with elevated levels during infection of potato by P. infestans.

Authors/Creators:Åsman, Anna and Vetukuri, Ramesh and Jahan, Sultana and Fogelqvist, Johan and Corcoran, Pádraic and Avrova, Anna and Whisson, Stephen and Dixelius, Christina
Title:Fragmentation of tRNA in Phytophthora infestans asexual life cycle stages and during host plant infection
Series/Journal:BMC Microbiology (1471-2180)
Year of publishing :2014
Volume:14
Page range:1-12
Number of Pages:12
Publisher:BioMed Central
ISSN:1471-2180
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:H Protection of plants and stored products > H20 Plant diseases
X Agricola extesions > X50 Chemistry
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Developmental Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
Agrovoc terms:transfer rna, infection, argonaute, phytophthora infestans
Keywords:argonaute, dicer, phytophthora infestans, potato, rna silencing, Small rna, tRF, tRNA
URN:NBN:urn:nbn:se:slu:epsilon-e-2626
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2626
Additional ID:
Type of IDID
Web of Science (WoS)000347355900001
ID Code:12146
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Plant Biology (from 140101)
Deposited By: SLUpub Connector
Deposited On:25 May 2015 12:29
Metadata Last Modified:13 Feb 2016 11:58

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