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Development of a genetic transformation method for seabuckthorn (Hippophae rhamnoides L.)

Sriskandarajah, Sridevy and Clapham, David and Lundquist, Per-Olof (2014). Development of a genetic transformation method for seabuckthorn (Hippophae rhamnoides L.). American Journal of Plant Sciences. 5:5, 528-534
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Official URL: http://dx.doi.org/10.4236/ajps.2014.55067

Abstract

Seabuckthorn (Hippophae rhamnoides L.) is a dioecious plant with berries containing highamounts of several bioactive compounds with nutritional and medicinal traits. It is also planted tocontrol soil erosion. A genetic transformation procedure will facilitate studies of the control ofplant development and interactions with symbionts and pathogens, and will provide a tool forplant breeding. Here, we present a particle bombardment method for transforming seabuckthorn.The early stages of induced adventitious shoots from roots were chosen as a novel target tissue forthe transformation procedure. The root system was bombarded with gold particles coated withplasmid pRT99gus containing genes for plant kanamycin resistance and for β-glucuronidase expression,and shoots were regenerated under kanamycin selection. PCR analysis of the regeneratedtransformed lines confirmed the presence of a 603 bp gus (uidA) gene fragment and a 1.5 kbfragment from the 35S promoter in three shoots from independent transformation events.

Authors/Creators:Sriskandarajah, Sridevy and Clapham, David and Lundquist, Per-Olof
Title:Development of a genetic transformation method for seabuckthorn (Hippophae rhamnoides L.)
Series/Journal:American Journal of Plant Sciences (2158-2742)
Year of publishing :2014
Depositing date:4 June 2015
Volume:5
Number:5
Page range:528-534
Number of Pages:6
Publisher:Scientific Research
ISSN:2158-2742
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
Agrovoc terms:genetic transformation, hippophae, plant genetics, breeding
Keywords:genetic transformation, plant breeding, plant genetics, hippophae
URN:NBN:urn:nbn:se:slu:epsilon-e-2629
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2629
Additional ID:
Type of IDID
DOI10.4236/ajps.2014.55067
ID Code:12148
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
NL - Faculty of Natural Resources and Agricultural Sciences (until 2013)
Department:(NL, NJ) > Department of Plant Biology (from 140101)
Deposited By: SLUpub Connector
Deposited On:04 Jun 2015 11:31
Metadata Last Modified:03 Aug 2015 18:14

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