Sriskandarajah, Sridevy and Clapham, David and Lundquist, Per-Olof
(2014).
Development of a genetic transformation method for seabuckthorn (Hippophae rhamnoides L.).
American Journal of Plant Sciences. 5
:5
, 528-534
[Journal article]
![]() |
PDF
- Published Version
Available under License Creative Commons Attribution. 847kB |
Official URL: http://dx.doi.org/10.4236/ajps.2014.55067
Abstract
Seabuckthorn (Hippophae rhamnoides L.) is a dioecious plant with berries containing highamounts of several bioactive compounds with nutritional and medicinal traits. It is also planted tocontrol soil erosion. A genetic transformation procedure will facilitate studies of the control ofplant development and interactions with symbionts and pathogens, and will provide a tool forplant breeding. Here, we present a particle bombardment method for transforming seabuckthorn.The early stages of induced adventitious shoots from roots were chosen as a novel target tissue forthe transformation procedure. The root system was bombarded with gold particles coated withplasmid pRT99gus containing genes for plant kanamycin resistance and for β-glucuronidase expression,and shoots were regenerated under kanamycin selection. PCR analysis of the regeneratedtransformed lines confirmed the presence of a 603 bp gus (uidA) gene fragment and a 1.5 kbfragment from the 35S promoter in three shoots from independent transformation events.
Authors/Creators: | Sriskandarajah, Sridevy and Clapham, David and Lundquist, Per-Olof | ||||
---|---|---|---|---|---|
Title: | Development of a genetic transformation method for seabuckthorn (Hippophae rhamnoides L.) | ||||
Series/Journal: | American Journal of Plant Sciences (2158-2742) | ||||
Year of publishing : | 2014 | ||||
Depositing date: | 4 June 2015 | ||||
Volume: | 5 | ||||
Number: | 5 | ||||
Page range: | 528-534 | ||||
Number of Pages: | 6 | ||||
Publisher: | Scientific Research | ||||
ISSN: | 2158-2742 | ||||
Language: | English | ||||
Publication Type: | Journal article | ||||
Refereed: | Yes | ||||
Article category: | Scientific peer reviewed | ||||
Version: | Published version | ||||
Copyright: | Creative Commons: Attribution 3.0 | ||||
Full Text Status: | Public | ||||
Agris subject categories.: | F Plant production > F30 Plant genetics and breeding | ||||
Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology | ||||
Agrovoc terms: | genetic transformation, hippophae, plant genetics, breeding | ||||
Keywords: | genetic transformation, plant breeding, plant genetics, hippophae | ||||
URN:NBN: | urn:nbn:se:slu:epsilon-e-2629 | ||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2629 | ||||
Additional ID: |
| ||||
ID Code: | 12148 | ||||
Faculty: | NJ - Fakulteten för naturresurser och jordbruksvetenskap NL - Faculty of Natural Resources and Agricultural Sciences (until 2013) | ||||
Department: | (NL, NJ) > Department of Plant Biology (from 140101) | ||||
Deposited By: | SLUpub Connector | ||||
Deposited On: | 04 Jun 2015 11:31 | ||||
Metadata Last Modified: | 09 Sep 2020 14:17 |
Repository Staff Only: item control page