Simanshu, Dhirendra K. and Hofius, Daniel and Brown, Rhoderick E.
(2014).
Arabidopsis accelerated cell death 11, ACD11, is a ceramide-1-phosphate transfer protein and intermediary regulator of phytoceramide levels.
Cell Reports. 6
:2
, 388–399
[Journal article]
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Available under License Creative Commons Attribution Non-commercial No Derivatives. 3MB |
Official URL: http://dx.doi.org/10.1016/j.celrep.2013.12.023
Abstract
The accelerated cell death 11 (acd11) mutant of Arabidopsis provides a genetic model for studying immune response activation and localized cellular suicide that halt pathogen spread during infection in plants. Here, we elucidate ACD11 structure and function and show that acd11 disruption dramatically alters the in vivo balance of sphingolipid mediators that regulate eukaryotic-programmed cell death. In acd11 mutants, normally low ceramide-1- phosphate (C1P) levels become elevated, but the relatively abundant cell death inducer phytoceramide rises acutely. ACD11 exhibits selective intermembrane transfer of C1P and phyto-C1P. Crystal structures establish C1P binding via a surface-localized, phosphate headgroup recognition center connected to an interior hydrophobic pocket that adaptively ensheaths lipid chains via a cleft-like gating mechanism. Point mutation mapping con- firms functional involvement of binding site residues. A p helix (p bulge) near the lipid binding cleft distinguishes apo-ACD11 from other GLTP folds. The global two-layer, a-helically dominated, ‘‘sandwich’’ topology displaying C1P-selective binding identifies ACD11 as the plant prototype of a GLTP fold subfamily.
Authors/Creators: | Simanshu, Dhirendra K. and Hofius, Daniel and Brown, Rhoderick E. | ||||||
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Title: | Arabidopsis accelerated cell death 11, ACD11, is a ceramide-1-phosphate transfer protein and intermediary regulator of phytoceramide levels | ||||||
Series/Journal: | Cell Reports (2211-1247) | ||||||
Year of publishing : | 2014 | ||||||
Volume: | 6 | ||||||
Number: | 2 | ||||||
Page range: | 388–399 | ||||||
Number of Pages: | 11 | ||||||
Publisher: | Elsevier | ||||||
ISSN: | 2211-1247 | ||||||
Language: | English | ||||||
Publication Type: | Journal article | ||||||
Refereed: | Yes | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution-Noncommercial-No Derivative Works 3.0 | ||||||
Full Text Status: | Public | ||||||
Agris subject categories.: | F Plant production > F30 Plant genetics and breeding | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Developmental Biology | ||||||
Agrovoc terms: | cell death, immune response, arabidopsis | ||||||
Keywords: | cell death, immune response, arabidopsis | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-e-2631 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2631 | ||||||
Additional ID: |
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ID Code: | 12158 | ||||||
Faculty: | NJ - Fakulteten för naturresurser och jordbruksvetenskap | ||||||
Department: | (NL, NJ) > Department of Plant Biology (from 140101) | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 25 May 2015 12:35 | ||||||
Metadata Last Modified: | 09 Sep 2020 14:17 |
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