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Potentially virulence-related extracellular proteins of Streptococcus equi

Lannergård, Jonas (2006). Potentially virulence-related extracellular proteins of Streptococcus equi. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2006:80
ISBN 91-576-7129-X
[Doctoral thesis]

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Equine strangles, a disease of the upper respiratory tract caused by the bacterium Streptococcus equi subspecies equi, is one of the most commonly diagnosed and serious diseases in horses. However, the molecular basis of S. equi subsp. equi infection is poorly understood and there are no safe and effective vaccines on the market. The main objective of this study was to identify and characterise extracellular proteins used by S. equi subsp. equi to initiate infection and cause disease. Extracellular proteins, which can be secreted or cell surface-located, play an important role in the initiation of infection and in continued bacterial survival inside the host. Adhesins are a specific class of virulence-related proteins that are used by the bacteria to attach to host tissues. This study focused on a number of cell-surface anchored proteins that specifically adhere to collagen and fibronectin, two major extracellular matrix proteins of vertebrates. The binding characteristics of the fibronectin-binding protein FNEB were compared to two previously studied fibronectin-binding proteins, FNE and SFS. A follow-up investigation showed that FNE and FNEB are part of a family of six similar proteins encoded by S. equi subsp. equi. One of the four novel proteins identified (FNEE) was shown to bind to fibronectin and all four to collagen. In another study, a secreted immunoglobulin-specific protease was characterised. This enzyme could be used by the bacteria to interfere with the immune response of the infected horse. A useful tool in this research was the public S. equi subsp. equi genome database, which can be used to identify homologues to virulence-related proteins of other pathogens. An applied objective of the present study was to identify potential components for a future vaccine against strangles. One of the collagen-binding proteins characterised (CNE) is currently being used in vaccination trials as a component of a protein subunit vaccine for horses.

Authors/Creators:Lannergård, Jonas
Title:Potentially virulence-related extracellular proteins of Streptococcus equi
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :2006
Number of Pages:46
ALLI. Lannergård J., Frykberg L. & Guss B. 2003. CNE, a collagenbinding protein of Streptococcus equi. FEMS Microbiology Letters 222: 69-74. II. Lannergård J., Flock M., Johansson S., Flock J-I. & Guss B. 2005. Studies of fibronectin-binding proteins of Streptococcus equi. Infection and Immunity 73: 7243-51. III. Lannergård J. & Guss B. 2006. IdeE, an IgG-endopeptidase of Streptococcus equi ssp. equi. FEMS Microbiology Letters 262: 230-235. IV. Lannergård J. & Guss B. FNE belongs to a novel family of fibronectin-binding and collagen-binding proteins of Streptococcus equi subsp. equi. (Manuscript)
Place of Publication:Uppsala
ISBN for printed version:91-576-7129-X
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:streptococcus equi, streptococcus zooepidemicus, bacterioses, pathogenicity, glycoproteins, collagen, immunoglobulins
Keywords:Streptococcus equi, Streptococcus zooepidemicus, strangles, virulence, fibronectin, collagen, extracellular matrix, adhesin, immunoglobulin
Permanent URL:
ID Code:1224
Department:(NL, NJ) > Dept. of Microbiology (until 161231)
Deposited By: Jonas Lannergård
Deposited On:04 Oct 2006 00:00
Metadata Last Modified:02 Dec 2014 10:10

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