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NMR metabolomics and lipid analyses in salmonid tissue

response to alternative feeds

Wagner, Liane (2015). NMR metabolomics and lipid analyses in salmonid tissue. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2015:62
ISBN 978-91-576-8322-9
eISBN 978-91-576-8323-6
[Doctoral thesis]

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Abstract

Salmonids are fatty fish and contain high amounts of omega-3 polyunsaturated fatty acids, beneficial in human nutrition. However, the aquaculture industry has expanded greatly in recent decades, increasing demand for fishmeal (FM) and fish oil (FO) for fish feed, while the supply of fish raw materials has remained static or declined. This has created a need for alternative sustainable raw materials for fish feeds.

This thesis examined the effect of replacing FM or FO with alternative plant (vegetable oils (VO), sesamin), microbial (zygomycete-, yeast fungi) and marine (krill, mussel) raw materials on growth performance, lipid content, fatty acid composition, lipid classes and fish metabolism in Atlantic salmon (Salmo salar) and Arctic charr (Salvelinus alpinus).

There was a significant negative effect on growth performance when sesamin, zygomycete fungi and extracted yeast were included in fish diets. Replacement of FM or FO with alternative materials affected the lipid profile in fish tissues, e.g. compared with the control, docosahexaenoic acid (DHA) level was higher in fish fed krill meal and mussel meal and lower in fish fed VO, but unaffected in fish fed extracted and non-extracted yeast and sesamin. Zygomycete fungi meal inclusion decreased fish lipid content, thereby increasing DHA by 54% of total lipid content. Metabolomics analyses revealed an effect on several metabolites depending on the diet. Inclusion of sesamin increased the levels of some metabolites associated with energy metabolism, suggesting metabolic disturbance in the fish. Replacement of FM with non-extracted yeast affected liver metabolites, while extracted yeast mainly increased muscle metabolite levels. These increases suggested that the test ingredients produced a significant response in fish muscle metabolism. The altered metabolic fingerprint in fish fed krill and mussel meal diets compared with FM suggested differences in ingredient composition and physiological response to the diets.

These results provide new information on the effects of non-traditional ingredients on fish metabolism, e.g. lipid-, single-carbon metabolism and tricarboxylic acid cycle. More studies are needed to identify the biochemical mechanisms involved and commercialise alternative fish feed materials.

Authors/Creators:Wagner, Liane
Title:NMR metabolomics and lipid analyses in salmonid tissue
Subtitle:response to alternative feeds
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :2015
Depositing date:19 May 2015
Volume:2015:62
Number of Pages:67
Papers/manuscripts:
NumberReferences
ISchiller Vestergren A.*, Wagner, L.*, Pickova, J., Rosenlund, G., Kamal-Eldin, A. & Trattner, S. (2012). Sesamin modulates gene expression without corresponding effect on fatty acids in Atlantic salmon (Salmo salar L.). Lipids 47(9), 897-911. *Contributed equally.
IIWagner, L., Trattner, S., Pickova, J., Goméz-Requeni, P. & Moazzami, A.A. (2014). 1H NMR-based metabolomics studies on the effect of sesamin in Atlantic salmon (Salmo salar). Food Chemistry 147(0), 98-105.
IIIIII Wagner, L., Gómez-Requeni, P., Moazzami, A.A., Pickova, J., Vidakovic, A., Langeland, M., Kiessling, A. & Lundh T. (2015). 1H NMR-based metabolomics and lipid profile analysis of muscle and liver of Arctic charr (Salvelinus alpinus) fed microbial or mussel meal. (submitted to British Journal of Nutrition)
IVWagner, L., Pan, J., Didone, R., Müllner, E., Brännäs, E., Pickova, J. & Moazzami, A.A. (2015). Fatty acid analysis and 1H NMR-based metabolomics study of Arctic charr (Salvelinus alpinus) fed with marine sources. (manuscript)
Place of Publication:Uppsala
Publisher:Department of Food Science, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8322-9
ISBN for electronic version:978-91-576-8323-6
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:Q Food science > Q04 Food composition
Q Food science > Q54 Feed composition
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Food Science
Agrovoc terms:salmo salar, chars, fish feeding, metabolism, fatty acids, lignans, docosahexaenoic acid, krill, microorganisms
Keywords:Atlantic salmon, Arctic charr, sesamin, krill, mussel, microorganism, zygomycetes fungi, yeast, fish metabolism, fatty acids, DHA
URN:NBN:urn:nbn:se:slu:epsilon-e-2669
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2669
ID Code:12241
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Food Science (until 161231)
Deposited By: Liane Wagner
Deposited On:20 May 2015 08:41
Metadata Last Modified:01 Jun 2015 07:38

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