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Recombinant feline coronaviruses as vaccine candidates confer protection in SPF but not in conventional cats

Balint, Adam and Farsang, Attila and Szeredi, Levente and Zadori, Zoltan and Belak, Sandor (2014). Recombinant feline coronaviruses as vaccine candidates confer protection in SPF but not in conventional cats. Veterinary microbiology. 169:3-4, 154-162
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Official URL: http://dx.doi.org/10.1016/j.vetmic.2013.10.015

Abstract

Feline infectious peritonitis virus (FIPV) is a major pathogen of Felidae. Despite the extensive efforts taken in the past decades, development of the "ideal" live attenuated FIPV vaccine was not successful yet. In the present study, we provide data of immunisation experiments with a recombinant FCoV pair differing only in the truncation (PBFIPV-DF-2) or completion (PBFIPV-DF-2-R3i) of their ORF3abc regions. In our previous in vivo studies, these viruses proved to show the characters of low virulent or avirulent FCoV phenotypes, respectively. Therefore, we hypothesised the ability of these viruses, as possible vaccine candidates, in conferring protection in specific pathogen free (SPF) Domestic Shorthair as well as in conventional purebred British Shorthair cats. In SPF cats, after two oronasal and two intramuscular vaccinations with two weeks intervals, both vaccine candidates provided 100% protection against lethal homologous challenge with the highly virulent FIPV DF-2 strain. In contrast, the conventional purebred British Shorthair cats did not develop protection when they were immunised with the same vaccination regimes. In these groups 100% of the PBFIPV-DF-2-R3i immunised animals developed antibody-dependent enhancement (ADE). Prolonged survival was observed in 40% of the animals, while 60% showed fulminant disease course. Genetic and more probably immunological differences between the SPF and non-SPF purebred kittens can explain the different outcome of the vaccination experiment. Our data highlight the diverse immune responses between SPF and conventional cats and suggest a decisive role of previous infection by heterologous causative agents in the outcome of the vaccination against FIP.

Authors/Creators:Balint, Adam and Farsang, Attila and Szeredi, Levente and Zadori, Zoltan and Belak, Sandor
Title:Recombinant feline coronaviruses as vaccine candidates confer protection in SPF but not in conventional cats
Series/Journal:Veterinary microbiology (0378-1135)
Year of publishing :2014
Volume:169
Number:3-4
Page range:154-162
Number of Pages:9
Publisher:Elsevier
ISSN:0378-1135
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:L Animal production > L70 Veterinary science and hygiene - General aspects
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Medical Bioscience
Agrovoc terms:feline infectious peritonitis, viruses, vaccine, immunization
Keywords:feline coronavirus, feline infectious peritonitis, vaccine, protection
URN:NBN:urn:nbn:se:slu:epsilon-e-2813
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2813
Additional ID:
Type of IDID
Web of Science (WoS)000333493300005
DOI10.1016/j.vetmic.2013.10.015
ID Code:12426
Deposited By: SLUpub Connector
Deposited On:29 Jul 2015 13:11
Metadata Last Modified:22 Feb 2016 07:17

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