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High prevalence of hepatitis e virus in swedish moose - A phylogenetic characterization and comparison of the virus from different regions

Lin, Jay and Lin, Jay and Karlsson, Marie and Olofson, Ann-Sophie and Belak, Sandor and Malmsten, Jonas and Dalin, Anne-Marie and Widen, Frederik and Norder, Heléne (2015). High prevalence of hepatitis e virus in swedish moose - A phylogenetic characterization and comparison of the virus from different regions. PloS one. 10:4, 1-14
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Official URL: http://dx.doi.org/10.1371/journal.pone.0122102

Abstract

BackgroundHepatitis E virus (HEV) infects a range of species, including humans, pigs, wild boars and deer. Zoonotic transmission may contribute to the high HEV seroprevalence in the human population of many countries. A novel divergent HEV from moose (Alces alces) in Sweden was recently identified by partial genome sequencing. Since only one strain was found, its classification within the HEV family, prevalence in moose and zoonotic potential was unclear. We therefore investigated samples from 231 moose in seven Swedish counties for HEV, and sequenced a near complete moose HEV genome. Phylogenetic analysis to classify this virus within the family Hepeviridae and to explore potential host specific determinants was performed.Methods and FindingsThe HEV prevalence of moose was determined by PCR (marker for active infection) and serological assays (marker of past infection) of sera and 51 fecal samples from 231 Swedish moose. Markers of active and past infection were found in 67 (29%) animals, while 34 (15%) were positive for HEV RNA, 43 (19%) were seropositive for anti-HEV antibodies, and 10 (4%) had both markers. The number of young individuals positive for HEV RNA was larger than for older individuals, and the number of anti-HEV antibody positive individuals increased with age. The high throughput sequenced moose HEV genome was 35-60% identical to existing HEVs. Partial ORF1 sequences from 13 moose strains showed high similarity among them, forming a distinct monophyletic clade with a common ancestor to HEV genotype 1-6 group, which includes members known for zoonotic transmission.ConclusionsThis study demonstrates a high frequency of HEV in moose in Sweden, with markers of current and past infection demonstrated in 30% of the animals. Moose is thus an important animal reservoir of HEV. The phylogenetic relationship demonstrated that the moose HEV belonged to the genotype 1-6 group, which includes strains that also infect humans, and therefore may signify a potential for zoonotic transmission of this HEV.

Authors/Creators:Lin, Jay and Lin, Jay and Karlsson, Marie and Olofson, Ann-Sophie and Belak, Sandor and Malmsten, Jonas and Dalin, Anne-Marie and Widen, Frederik and Norder, Heléne
Title:High prevalence of hepatitis e virus in swedish moose - A phylogenetic characterization and comparison of the virus from different regions
Series/Journal:PloS one (1932-6203)
Year of publishing :2015
Volume:10
Number:4
Page range:1-14
Number of Pages:14
Publisher:Public Library of Science
ISSN:1932-6203
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:L Animal production > L70 Veterinary science and hygiene - General aspects
L Animal production > L73 Animal diseases
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Medical Bioscience
Agrovoc terms:hepatitis, elks, genomes, antibodies, sweden
Keywords:hepatitis, elks, genomes, antibodies, sweden
URN:NBN:urn:nbn:se:slu:epsilon-e-2821
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2821
Additional ID:
Type of IDID
Web of Science (WoS)000353332000010
ID Code:12437
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(LTJ, LTV) > Department of Biosystems and Technology (from 130101)
(VH) > Department of Biosystems and Technology (from 130101)

(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:30 Jul 2015 13:45
Metadata Last Modified:18 Feb 2016 20:17

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